Pneumolysin generates multiple conductance pores in the membrane of nucleated cells
Pneumolysin generates multiple conductance pores in the membrane of nucleated cells
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DOI：
10.1016/j.bbrc.2008.01.151
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发表时间：
2008-04-11
影响因子：
3.1
通讯作者：
Andrew, Peter W.
中科院分区：
文献类型：
--
作者：
El-Rachkidy, Rana G.;Davies, Noel W.;Andrew, Peter W.
膜片钳被用来调查，第一次，由胆固醇依赖性溶细胞素在有核细胞的膜，而不是在人工膜如前所述，形成的孔的电生理特性。CHO细胞膜的内面向外补丁中的溶血素孔具有广泛的电导范围，分为小电导（200 pS和1 nS）。肺炎球菌溶血素的裂解缺陷突变体（W433F）诱导类似的通道谱，但有更多的小通道比野生型（WT）毒素和事件的数量减少。WT毒素在给予质膜内表面时也诱导通道。(c)2008年爱思唯尔公司All rights reserved.
Patch clamping was used to investigate, for the first time, the electrophysiological properties of pores formed by a cholesterol-dependent cytolysin in the membrane of nucleated cells rather than in artificial membranes as previously. Pneumolysin pores in inside-out patches of CHO cell membranes had a broad range of conductance classified into small (200 pS and 1 nS). A lytic-deficient mutant of pneumolysin (W433F) induced a similar spectrum of channels but there were more small channels than with wild-type (WT) toxin and the number of events was decreased. The WT toxin also induced channels when given to the inside surface of the plasma membrane. (c) 2008 Elsevier Inc. All rights reserved.