GABA and nitric oxide synthase immunoreactivities are colocalized in a subset of inhibitory motor neurons of the guinea-pig small intestine
GABA and nitric oxide synthase immunoreactivities are colocalized in a subset of inhibitory motor neurons of the guinea-pig small intestine
复制标题
GABA 和一氧化氮合酶免疫反应性共定位于豚鼠小肠的抑制性运动神经元子集中
作者：
S. Williamson;S. Pompolo;J. Furness
摘要：用免疫荧光同时标记法研究了豚鼠小肠神经细胞和纤维中γ-氨基丁酸免疫反应与一氧化氮合酶、血管活性肠肽和速激肽的共存模式。GABA-IR神经元胞体位于肌间神经丛内，肌间神经节内曲张纤维支配环肌和纵肌，但不形成细胞周末梢。GABA-IR神经细胞占肌间神经节神经细胞总数的4-5%。在GABA-IR的肌间神经细胞中，约85%为NOS-IR，在两个肌层的GABA-IR神经纤维中，约75%为NOS-IR。相反，20%的NOS-IR神经细胞为GABA-IR。支配环行肌的GABA免疫反应阳性神经纤维约占6%，而支配纵行肌的GABA免疫反应阳性神经纤维约占6%。供应环状肌的GABA-IR纤维多为VIP-IR，而供应纵行肌的GABA-IR纤维则无一例。根据本研究和前人对肠神经元投射的研究，得出结论：豚鼠小肠内的大多数GABA-IR神经元是抑制性运动神经元，也含有NOS-IR。一小部分代表支配环肌的肛门定向兴奋性运动神经元，也是TK免疫反应阳性的神经元。
Abstract.Simultaneous immunofluorescence labelling was used to determine the patterns of colocalization of immunoreactivity for γ-aminobutyric acid (GABA-IR) with immunoreactivity for nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP) and tachykinins (TK) in nerve cells and fibres of the guinea-pig small intestine. GABA-IR nerve cell bodies were located in the myenteric plexus and varicose fibres innervated the circular and longitudinal muscle, but did not form pericellular endings in the myenteric ganglia. GABA-IR nerve cells comprised 4–5% of all nerve cells in the myenteric ganglia. Of GABA-IR myenteric nerve cells, about 85% had NOS-IR and of GABA-IR nerve fibres in both muscle layers, about 75% were NOS-IR. Conversely, 20% of NOS-IR nerve cells were GABA-IR. About 6% of GABA-IR nerve fibres innervating the circular muscle, but none innervating the longitudinal muscle, were TK-IR. Most GABA-IR fibres supplying the circular muscle, but none of those supplying the longitudinal muscle, were VIP-IR. From this study, and previous studies of projections of enteric neurons, it is concluded that most GABA-IR neurons in the guinea-pig small intestine are inhibitory motor neurons that also contain NOS-IR. A small proportion represents anally directed excitatory motor neurons that innervate the circular muscle and are also immunoreactive for TK.
一氧化氮合成的拮抗剂抑制豚鼠回肠中神经介导的纵向肌肉的松弛。
DOI：
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发表时间：
1992
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Osthaus,LE;Galligan,JJ
通讯作者：
Galligan,JJ