A joint genomewide association analysis of pig leg weakness and its related traits in an F2 population and a Sutai population
A joint genomewide association analysis of pig leg weakness and its related traits in an F2 population and a Sutai population
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DOI：
10.2527/jas.2012-6210
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发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Huang, L. S.
中科院分区：
文献类型：
--
作者：
Guo, Y. M.;Zhang, X. F.;Huang, L. S.
为了定位猪腿弱相关性状的基因座，在219 +/- 18 d时记录了白色杜洛克×二花脸F2杂交群体和中国苏太猪群体的腿评分和步态评分。在240 ± 3日龄屠宰后，从2个群体的右前腿解剖肱二头肌，并测量其长度和重量。使用猪SNP 60 BeadChip对2个群体进行基因分型，并分别和联合对它们进行全基因组关联研究。在3个群体中共检测到12个显著性位点，其中6个在SSC 7和SSCX上达到1%显著水平，2个在SSC 7和SSCX上达到5%显著水平。除1个在SSC 5上的前腿步态评分位点为首次报道外，其余位点均证实了前人的QTL定位结果。最突出的位点被确定在一个2.15 Mb的连锁不平衡块上的SSC 7腿无力和肱二头肌肌肉的增长，这是值得进一步调查。在苏台猪群体中发现的显著SNP可直接用于标记辅助选择，以改善苏台猪的腿健全性。正如预期的那样，与单个总体相比，在组合总体中识别重要区域通常更有效。据我们所知，这是第一个全基因组关联研究猪肱二头肌的重量和长度。
To map genomic loci for leg weakness-related traits in pigs, leg scores and gait scores were recorded at 219 +/- 18 d in a White Duroc x Erhualian F-2 intercross population and a Chinese Sutai population. The biceps brachii muscle was dissected from the right front leg and its length and weight were measured after slaughter at 240 +/- 3 d in the 2 populations. The 2 populations were genotyped using the Porcine SNP60 BeadChip, and genomewide association studies were performed on them separately and jointly. A total of 12 significant loci were detected in the 3 populations, including 6 at the 1% genomewide significant level on SSC7 and SSCX and 2 at the 5% genomewide significant level on SSC7 and SSCX. All of them confirmed the previous QTL findings except 1 locus for gait score of front legs on SSC5, which was reported for the first time. The most prominent locus was identified in a 2.15 Mb linkage disequilibrium block on SSC7 for both leg weakness and the growth of the biceps brachii muscle, which is worth further investigation. The significant SNP identified in the Sutai population could directly be explored in marker-assisted selection to improve leg soundness of the Sutai pig. As expected, it is generally more powerful to identify significant regions in the combined population compared with a single population. To our knowledge, this was the first genomewide association study for weight and length of the biceps brachii muscle in pigs.