Measuring the absolute abundance of the Smad transcription factors using quantitative immunoblotting.
Measuring the absolute abundance of the Smad transcription factors using quantitative immunoblotting.
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DOI：
10.1007/978-1-60761-738-9_22
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发表时间：
2010
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在系统生物学时代，生物学家试图量化实验处理样品中分子的绝对数量。免疫印迹仍然是评估不同样品中蛋白质水平相对差异的一种选择技术。在这里，我们讨论了如何利用免疫印迹来估计每个细胞中Smad转录因子分子的数量。我们专注于描述分析数据所需的计算。我们的方法一般适用于其他细胞蛋白的定量。
In the age of systems biology, biologists seek to quantify the absolute number of molecules in experimentally treated samples. Immunoblotting remains a technique of choice for assessing the relative differences between the protein levels in different samples. Here we discuss how to exploit immunoblotting for estimating the number of Smad transcription factor molecules per cell. We focus on describing the calculations needed to analyze the data. Our methods are generally applicable to the quantification of other cellular proteins.