Effects of spaceflight on innate immune function and antioxidant gene expression
Effects of spaceflight on innate immune function and antioxidant gene expression
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DOI：
10.1152/japplphysiol.91361.2008
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发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Pecaut, Michael J.
中科院分区：
文献类型：
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作者：
Baqai, Farnaz P.;Gridley, Daila S.;Pecaut, Michael J.
Baqai FP，Gridley DS，斯莱特JM，Luo-Owen X，Stodieck LS，Ferguson V，Chapes SK，Pecaut MJ.航天对天然免疫功能及抗氧化基因表达的影响。J Appl Physiol 106：1935-1942，2009.首次发表于2009年4月2日; doi：10.1152/japplphysiol.91361.2008。由于心理压力、辐射和重力降低，航天条件对许多生理功能产生重大影响。为了探索飞行环境对免疫力的影响，将C57 BL/6 NTac小鼠空运到为期13天的航天飞机使命（STS-118）中。与地面（GRD）对照组相比，动物对飞行的反应是肝脏、脾脏和胸腺质量减少（P < 0.005）。脾淋巴细胞、单核/巨噬细胞和粒细胞计数在飞行（FLT）小鼠中显著降低（P < 0.05）。与GRD小鼠相比，FLT小鼠脾细胞自发性囊胚形成增加，但对大肠杆菌B细胞有丝分裂原脂多糖（LPS）的反应降低（P < 0.05）。FLT小鼠脾细胞经LPS刺激后分泌IL-6和IL-10增加（P < 0.05），但TNF-α无明显变化。最后，许多负责清除活性氧的基因在飞行后上调。这些数据表明，暴露于航天环境可以增加抗炎机制，并改变对LPS的离体反应，LPS是一种与脓毒性休克和突出的Th 1反应相关的细菌产物。
Baqai FP, Gridley DS, Slater JM, Luo-Owen X, Stodieck LS, Ferguson V, Chapes SK, Pecaut MJ. Effects of spaceflight on innate immune function and antioxidant gene expression. J Appl Physiol 106: 1935-1942, 2009. First published April 2, 2009; doi:10.1152/japplphysiol.91361.2008.-Spaceflight conditions have a significant impact on a number of physiological functions due to psychological stress, radiation, and reduced gravity. To explore the effect of the flight environment on immunity, C57BL/6NTac mice were flown on a 13-day space shuttle mission (STS-118). In response to flight, animals had a reduction in liver, spleen, and thymus masses compared with ground (GRD) controls (P < 0.005). Splenic lymphocyte, monocyte/macrophage, and granulocyte counts were significantly reduced in the flight (FLT) mice (P < 0.05). Although spontaneous blastogenesis of splenocytes in FLT mice was increased, response to lipopolysaccharide (LPS), a B-cell mitogen derived from Escherichia coli, was decreased compared with GRD mice (P < 0.05). Secretion of IL-6 and IL-10, but not TNF-alpha, by LPS-stimulated splenocytes was increased in FLT mice (P < 0.05). Finally, many of the genes responsible for scavenging reactive oxygen species were upregulated after flight. These data indicate that exposure to the spaceflight environment can increase anti-inflammatory mechanisms and change the ex vivo response to LPS, a bacterial product associated with septic shock and a prominent Th1 response.