Native form of endothelin receptor in human placental membranes.
Native form of endothelin receptor in human placental membranes.
复制标题
人胎盘膜中内皮素受体的天然形式。
DOI：
10.1016/0006-291x(90)91762-h
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发表时间：
1990
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Nakajo,S;Sugiura,M;Inagami,T
人们对内皮素受体的天然形式知之甚少。为了阐明其功能和结构性质，我们用温和的洗涤剂CHAPS和洋地黄素以活性形式溶解了人胎盘上的受体，结果表明它能够以特定的方式与125I-内皮素-1结合，其最适pH在6-8之间，而报道的最适pH为4。在0.2%(W V)洋地黄素存在下，通过凝胶过滤估计受体的相对分子质量为340,000。当溶解的膜在凝胶过滤前用125I-ET-1标记时，放射性配体也迁移到对应于340 kDa蛋白的位置。这些结果表明，人胎盘内皮素受体的天然形式是一个340 kDa的蛋白。
Little is known about the native form of endothelin receptor. To clarify its functional and structural properties, we solubilized the receptor from human placenta in an active form using mild detergents CHAPS and digitonin and showed that it is able to bind 125 I-endothelin-1 in a specific manner, with a pH optimum between 6 and 8 in contrast to a reported pH optimum of 4. The molecular weight of the receptor was estimated as 340,000 by gel filtration of the solubilized membrane in the presence of 0.2%(w v) digitonin. When the solubilized membranes were labeled with 125 I-endothelin-1 prior to gel filtration, the radioactive ligand also migrated in the position corresponding to a 340 kDa protein. These results indicated that the native form of endothelin receptor in human placenta is a 340 kDa protein.