Twin research shows glycan changes are more susceptible to environmental factors than their carrier glycoproteins
Twin research shows glycan changes are more susceptible to environmental factors than their carrier glycoproteins
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DOI：
10.1007/s10719-023-10099-7
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发表时间：
2023-02-14
影响因子：
3
通讯作者：
Miyoshi，Eiji
中科院分区：
文献类型：
--
作者：
Asuka，Tatsuya;Kamada，Yoshihiro;Miyoshi，Eiji
蛋白质糖基化的变化在临床上用作生物标志物。在本研究中，我们采用双胞胎队列研究遗传和环境因素对糖蛋白聚糖修饰的贡献。Mac-2结合蛋白（Mac-2 bp）、触珠蛋白（Hp）及其糖基化形式是肝纤维化和癌症的生物标志物。使用来自107对没有临床信息的双胞胎对的血清作为Mac-2 bp和Mac-2 bp糖基化异构体（M2 BPGi）测定的训练队列。作为验证队列，22对双胞胎被纳入研究。对于每对双胞胎来说，其中一名双胞胎被诊断出患有肝脏或胰腺疾病。对于训练队列，血清Mac-2 bp和M2 BPGi水平在双胞胎血清中与随机序列的相关比分别为0.30和0.018。验证队列中双胞胎对之间的血清Mac-2 bp和M2 BPGi水平的相关比分别为0.75和0.35。相反，血清Hp和岩藻糖基化结合珠蛋白（Fuc-Hp）水平之间的相关系数分别为0.49和0.16，肝脏和胰腺疾病的双胞胎血清。虽然糖蛋白的血清蛋白水平易受遗传因素影响，但这些糖蛋白的特征性聚糖变化更易受环境因素影响，包括肝脏和胰腺疾病。
Changes in protein glycosylation are clinically used as biomarkers. In the present study, we employed a twin cohort to investigate the contributions of genetic and environmental factors to glycan modifications of glycoproteins. Mac-2 binding protein (Mac-2 bp), haptoglobin (Hp), and their glycosylated forms are liver fibrosis and cancer biomarkers. Sera from 107 twin pairs without clinical information were used as a training cohort for the Mac-2 bp and Mac-2 bp glycosylation isomer (M2BPGi) assay. As a validation cohort, 22 twin pairs were enrolled in the study. For each twin pair, one twin was diagnosed with liver or pancreatic disease. For the training cohort, the correlation ratios of serum Mac-2 bp and M2BPGi levels in twin sera with random sequences were 0.30 and 0.018, respectively. The correlation ratios between twin pairs in the validation cohort for serum Mac-2 bp and M2BPGi levels were 0.75 and 0.35, respectively. In contrast, correlation ratios of serum Hp and fucosylated haptoglobin (Fuc-Hp) levels between twin sera with liver and pancreatic disease were 0.49 and 0.16, respectively. Although serum protein levels of glycoproteins are susceptible to genetic factors, characteristic glycan changes of these glycoproteins are more susceptible to environmental factors, including liver and pancreatic disease.Graphical abstract