Genome-wide amplifications caused by chromosomal rearrangements play a major role in the adaptive evolution of natural yeast.
Genome-wide amplifications caused by chromosomal rearrangements play a major role in the adaptive evolution of natural yeast.
复制标题
由染色体重排引起的全基因组扩增在天然酵母的适应性进化中发挥着重要作用。
DOI：
10.1093/genetics/165.4.1745
复制
发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Young,EltonT
中科院分区：
文献类型：
--
作者：
Infante,JuanJ;Dombek,KennethM;Rebordinos,Laureana;Cantoral,JesúsM;Young,EltonT
总的染色体重排对适应性进化的相对重要性还没有得到精确的定义。酿酒糖酵母菌株在非常严格的环境中进化到高度特化，为分子进化研究提供了显著的优势。利用DNA微阵列技术，我们比较了s的两个突出变体的基因组。酿酒酵母株。这些菌株在116个基因组区域（占基因组的38%）的DNA拷贝数上彼此不同。在大多数情况下，这些区域是由重复序列或先前描述为双链断裂发生区域的其他重组热点组成的扩增子。在扩增子中存在赋予花菌特定特征的基因，支持染色体重排作为适应性进化inS的主要机制的作用。酵母。我们提出，非等位基因相互作用被乙醇和乙醛诱导的染色体DNA双链断裂增强，这些断裂通过产生总体染色体重排的途径得到修复。染色体进化的这一机制也可以解释在floryeast中显示的性隔离。
The relative importance of gross chromosomal rearrangements to adaptive evolution has not been precisely defined. TheSaccharomyces cerevisiae floryeast strains offer significant advantages for the study of molecular evolution since they have recently evolved to a high degree of specialization in a very restrictive environment. Using DNA microarray technology, we have compared the genomes of two prominent variants ofS. cerevisiae floryeast strains. The strains differ from one another in the DNA copy number of 116 genomic regions that comprise 38% of the genome. In most cases, these regions are amplicons flanked by repeated sequences or other recombination hotspots previously described as regions where double-strand breaks occur. The presence of genes that confer specific characteristics to thefloryeast within the amplicons supports the role of chromosomal rearrangements as a major mechanism of adaptive evolution inS. cerevisiae. We propose that nonallelic interactions are enhanced by ethanol- and acetaldehyde-induced double-strand breaks in the chromosomal DNA, which are repaired by pathways that yield gross chromosomal rearrangements. This mechanism of chromosomal evolution could also account for the sexual isolation shown among thefloryeast.