Cytotoxicity of misonidazole and DNA damage in hypoxic mammalian cells.
Cytotoxicity of misonidazole and DNA damage in hypoxic mammalian cells.
复制标题
米索硝唑的细胞毒性和缺氧哺乳动物细胞中的 DNA 损伤。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
L. Skarsgard
中科院分区：
文献类型：
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作者：
B. Palcic;L. Skarsgard
研究了米索硝唑对不同类型哺乳动物细胞的集落形成能力和DNA单链断裂的影响。观察到细胞失活和DNA损伤之间的相关性。如果在暴露后，将细胞从药物中清洗出来，然后在37 ℃的生长培养基中孵育，观察到存活的细胞修复其DNA，使沉降曲线与未暴露的细胞相同。然而，在非存活细胞中，发现DNA在暴露后孵育中进一步降解。
The loss of colony-forming ability and the yield of DNA single strand breaks were studied following exposure of various mammalian cell types to misonidazole. A correlation was observed between cell inactivation and DNA damage. If, after exposure, the cells were washed free of the drug and then incubated at 37 degree C in growth medium, it was observed that surviving cells repaired their DNA to the point that sedimentation profiles were identical to those of unexposed cells. In nonsurviving cells, however, the DNA was found to be further degraded with post-exposure incubation.