Late-Emigrating Trunk Neural Crest Cells in Turtle Embryos Generate an Osteogenic Ectomesenchyme in the Plastron
Late-Emigrating Trunk Neural Crest Cells in Turtle Embryos Generate an Osteogenic Ectomesenchyme in the Plastron
复制标题
DOI：
10.1002/dvdy.24018
复制
发表时间：
2013-11-01
影响因子：
2.5
通讯作者：
Gilbert, Scott F.
中科院分区：
文献类型：
--
作者：
Cebra-Thomas, Judith A.;Terrell, Anne;Gilbert, Scott F.
研究背景：龟胸甲是由一层角质化的表皮覆盖在九层真皮骨上构成。它的发育起源一直存在争议;最近的证据表明，plastral骨来自躯干神经嵴细胞（NCC）。结果如下：这项研究扩展了观察，有一个海龟特有的，第二波的主干NCC分层和迁移后，原来的NCC已经到达目的地和分化。第二波通过免疫组织化学在整体安装和连续切片中证实，通过将DiI（1，1，di-octadecyl-3，3，3，3，-tetramethylindo-carbocyanine perchlorate）注射到神经管的管腔中并将标记的细胞追踪到腹板中，以及通过从较老的海龟胚胎中分离神经管并观察分层的NCC。这种后期的迁移产生了表达NCC标记物的外胚层间充质，并且可以被诱导以启动骨形成。结论：第二次迁移的NCC具有与早期NCC相似的性质，但也表达了颅NCC的特征性标志物。腹板间充质的大多数细胞表达神经嵴标记物，并且具有与颅面外间充质相似或相同的成骨分化能力。我们的证据支持这样的论点，即龟腹骨是来自一个后期移民人口的细胞来自躯干神经嵴。Developmental Dynamics 242：1223-1235，2013. (c)2013 Wiley Periodicals，Inc.
Background: The turtle plastron is composed of a keratinized epidermis overlying nine dermal bones. Its developmental origin has been controversial; recent evidence suggests that the plastral bones derive from trunk neural crest cells (NCCs). Results: This study extends the observations that there is a turtle-specific, second wave of trunk NCC delamination and migration, after the original NCCs have reached their destination and differentiated. This second wave was confirmed by immunohistochemistry in whole-mounts and serial sections, by injecting DiI (1,1, di-octadecyl-3,3,3,3,-tetramethylindo-carbocyanine perchlorate) into the lumen of the neural tube and tracing labeled cells into the plastron, and by isolating neural tubes from older turtle embryos and observing delaminating NCCs. This later migration gives rise to a plastral ectomesenchyme that expresses NCC markers and can be induced to initiate bone formation. Conclusions: The NCCs of this second migration have properties similar to those of the earlier NCCs, but also express markers characteristic of cranial NCCs. The majority of the cells of the plastron mesenchyme express neural crest markers, and have osteogenic differentiation capabilities that are similar or identical to craniofacial ectomesenchyme. Our evidence supports the contention that turtle plastron bones are derived from a late emigrating population of cells derived from the trunk neural crest. Developmental Dynamics 242:1223-1235, 2013. (c) 2013 Wiley Periodicals, Inc.