Probabilistic view of the transformation of cultured C3H/10T1/2 mouse embryo fibroblasts by 3-methylcholanthrene.
Probabilistic view of the transformation of cultured C3H/10T1/2 mouse embryo fibroblasts by 3-methylcholanthrene.
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3-甲基胆蒽转化培养的 C3H/10T1/2 小鼠胚胎成纤维细胞的概率视图。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
C. Heidelberger
中科院分区：
文献类型：
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作者：
A. Fernández;S. Mondal;C. Heidelberger
当用给定浓度的化学致癌物处理C3 H/10 T1/2细胞时，转化频率可以变化超过4个数量级，主要取决于接种的细胞数量。为了解释这一现象，我们已经开发了一个概率理论，在这个系统中的转换焦点的形成。我们将p1定义为细胞被致癌物处理激活的概率，p2定义为每代细胞中激活细胞转化的概率，p3定义为每代细胞中激活细胞失活的概率。我们推导出的方程：log（F/N）= log [2 p1 p2（1-- p3）/2（1 -- p3）-- 1]+ n log（1 -- p3）描述了病灶形成; F是致癌剂处理后每个培养皿中的平均位点数，N是汇合时培养皿中的细胞数，n是汇合的细胞代数。该方程已被实验验证;在单一浓度的3-甲基胆蒽下，p3 = 0.24和p1 p2 = 3.8 × 10（-6）。这种关系解释了以前无法解释的细胞密度对转化频率的影响。
When C3H/10T1/2 cells are treated with a given concentration of a chemical carcinogen, the transformation frequency can vary over 4 orders of magnitude, depending primarily upon the number of cells plated. To explain this phenomenon, we have developed a probabilistic theory of the formation of transformed foci in this system. We define p1 as the probability that a cell will be activated by carcinogen treatment, p2 as the probability per cell generation that an activated cell will be transformed, and p3 as the probability per cell generation that an activated cell will be deactivated. The equation we have derived: log (F/N) = log [2p1p2(1 -- p3)/2(1 -- p3) -- 1]+ n log (1 -- p3) describes focus formation; F is mean number of loci per dish after carcinogen treatment, N is number of cells in a dish at confluence, and n is number of cell generations to confluence. This equation has been verified experimentally; p3 = 0.24 and p1p2 = 3.8 X 10(-6) at a single concentration of 3-methylcholanthrene. This relationship explains previously inexplicable effects of cell density on transformation frequency.