Juvenile-hormone-dependent interaction of actin and spectrin is crucial for polymorphic differentiation of the larval honey bee ovary
Juvenile-hormone-dependent interaction of actin and spectrin is crucial for polymorphic differentiation of the larval honey bee ovary
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DOI：
10.1007/s00441-001-0490-y
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发表时间：
2002-02-01
影响因子：
3.6
通讯作者：
Hartfelder, K
中科院分区：
文献类型：
--
作者：
Capella, ICS;Hartfelder, K
蜜蜂工蜂卵巢细胞程序性死亡使变态期卵巢管数目从150-200个减少到10个以下。相反，蚁后卵巢中几乎所有的卵巢管都能存活到成虫阶段。多丝体的正确形成和持续存在被认为是卵巢管存活的关键因素。我们已经分析了F-肌动蛋白和α-血影蛋白的蜂王和工人幼虫的融合体，并在少年激素处理的工人幼虫的发展动态。小融合体含有肌动蛋白和血影蛋白可以检测到在两个种姓的四龄幼虫的卵巢。蜕皮后的第五龄，肌动蛋白血影蛋白协会坚持在扩大的纺锤体女王卵巢管。在工人，肌动蛋白从梭体和皮质α-血影蛋白解离。与凋亡标志物的出现相一致，在工蜂卵巢管中可检测到大的肌动蛋白聚集体。用保幼激素处理四龄工蜂幼虫可使卵巢管免于凋亡并维持肌动蛋白-血影蛋白的结合。幼激素依赖的肌动蛋白血影蛋白的相互作用，因此是最早的步骤之一，在一个多态性的卵巢分化。可塑性的卵巢管的数量作为一个结果，依赖于融合体的形成可能是一个更普遍的现象，在昆虫中，超出种姓多态性在高度eusocial Hyaltera。
Programmed cell death in the worker ovary of Apis mellifera reduces the number of ovarioles during metamorphosis from 150-200 primordia to less than 10. In contrast, practically all ovarioles in the ovary of queens survive to the adult stage. The correct formation and persistence of polyfusomes has been suggested as a critical factor for ovariole survival. We have analyzed the developmental dynamics of F-actin and alpha-spectrin in fusomes of queen and worker larvae, and in juvenile-hormone-treated worker larvae. Small fusomes containing actin and spectrin can be detected in the ovaries of fourth instar larvae in both castes. After molting to the fifth instar, the actin-spectrin association persists in the enlarged fusomes of queen ovarioles. In workers, actin dissociates from the fusomal and cortical alpha-spectrin. Coinciding with the appearance of apoptosis markers, large agglomerates of actin are detectable in worker ovarioles. Treatment of fourth-instar worker larvae with juvenile hormone rescues ovarioles from apoptosis and maintains the actin-spectrin association. Juvenile-hormone-dependent actin-spectrin interaction is thus one of the earliest steps in the differentiation of a polymorphic ovary. Plasticity in ovariole numbers as a result of hormone-dependent fusome formation may be a more widespread phenomenon in insects, extending beyond caste polymorphism in highly eusocial Hymenoptera.