Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries
Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries
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DOI：
10.1016/j.jcv.2012.05.010
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发表时间：
2012-09-01
影响因子：
8.8
通讯作者：
Cao-Lormeau, Van-Mai
中科院分区：
文献类型：
--
作者：
Aubry, Maite;Roche, Claudine;Cao-Lormeau, Van-Mai
背景资料：在太平洋岛屿国家，登革热的流行病学特征是单一登革热病毒血清型的长期传播。在一个岛国出现一种新的血清型，往往意味着该血清型的大规模爆发将在其他太平洋岛屿国家发生。目的：评估滤纸（FP）卡片作为一种通过标准邮寄方式在整个太平洋地区运输这种材料的方法，该卡片上已干燥了来自登革热疑似患者的全血或血清。在DENV-1和DENV-4传播期间，从新喀里多尼亚、沃利斯和富图纳群岛的患者中收集了22份FP干燥全血样本，并从马朱罗州雅普州的患者中收集了76份FP干燥血清（马歇尔群岛共和国）、汤加和斐济，在雅普州的DENV-2和马朱罗的DENV-4爆发之前和期间，在法属波利尼西亚的路易斯马拉德研究所通过血清型特异性RT-PCR检测DENV RNA的存在。在环境温度下运输超过三周后，可以通过各种RT-PCR程序在FP干燥的样品中确定DENV的血清型。在大多数情况下，包膜基因的测序基因分型的病毒也是possible.Conclusions：血清型和基因型的DENV可以确定从FP干燥的血清或全血样品运输超过数千公里，在环境，热带，温度。这种简单、低成本的病毒鉴定方法应在隔离和资源匮乏的环境中进行评估，以监测一系列重大病毒性疾病。(C)2012年由Elsevier B出版。诉
Background: In Pacific Island Countries (PICs) the epidemiology of dengue is characterized by long-term transmission of a single dengue virus (DENV) serotype. The emergence of a new serotype in one island country often indicates major outbreaks with this serotype will follow in other PICs.Objectives: Filter paper (FP) cards on which whole blood or serum from dengue suspected patients had been dried was evaluated as a method for transportation of this material by standard mail delivery throughout the Pacific.Study design: Twenty-two FP-dried whole blood samples collected from patients in New Caledonia and Wallis & Futuna Islands, during DENV-1 and DENV-4 transmission, and 76 FP-dried sera collected from patients in Yap State, Majuro (Republic of Marshall Islands), Tonga and Fiji, before and during outbreaks of DENV-2 in Yap State and DENV-4 in Majuro, were tested for the presence of DENV RNA, by serotype specific RT-PCR, at the Institut Louis Malarde in French Polynesia.Results: The serotype of DENV could be determined, by a variety of RT-PCR procedures, in the FP-dried samples after more than three weeks of transport at ambient temperatures. In most cases, the sequencing of the envelope gene to genotype the viruses also was possible.Conclusions: The serotype and genotype of DENV can be determined from FP-dried serum or whole blood samples transported over thousands of kilometers at ambient, tropical, temperatures. This simple and low-cost approach to virus identification should be evaluated in isolated and resource poor settings for surveillance for a range of significant viral diseases. (C) 2012 Published by Elsevier B. V.