Acinetobacter baumannii escape from neutrophil extracellular traps (NETs)
Acinetobacter baumannii escape from neutrophil extracellular traps (NETs)
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DOI：
10.1016/j.jiac.2014.08.032
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Ono, Yasuo
中科院分区：
文献类型：
--
作者：
Kamoshida, Go;Kikuchi-Ueda, Takane;Ono, Yasuo
鲍曼不动杆菌和铜绿假单胞菌是相同的需氧革兰氏阴性杆菌，通常是无害的，但会在受损的宿主中引起传染病。中性粒细胞在防止细菌胞外生长的感染性保护中发挥着关键作用。近年来，一种新的生物防御机制--中性粒细胞外陷阱(Nets)引起了人们的关注。在目前的研究中，我们调查了中性粒细胞对鲍曼不动杆菌和铜绿假单胞菌的反应性，重点是网络的形成。将中性粒细胞与鲍曼不动杆菌或铜绿假单胞菌共同培养，进行DNA、组蛋白和中性粒细胞弹性蛋白酶染色，观察中性粒细胞网状结构的形成。经鲍曼不动杆菌刺激的中性粒细胞虽有扩张，但其形态仍保持不变，细胞核与未受刺激的中性粒细胞一样清晰可见。然而，被铜绿假单胞菌刺激的中性粒细胞不能维持其细胞形态，细胞核被DNA、组蛋白和中性粒细胞弹性蛋白酶释放到细胞外空间而破坏。这些结果表明，与铜绿假单胞菌相比，鲍曼不动杆菌不能诱导网状形成。此外，我们还检测了中性粒细胞中髓过氧化物酶(MPO)、活性氧(ROS)和超氧化物歧化酶(O2)的表达，发现这些在铜绿假单胞菌刺激的中性粒细胞中的表达强于鲍曼不动杆菌刺激的中性粒细胞。此外，与铜绿假单胞菌不同，鲍曼不动杆菌不会被中性粒细胞杀死。在这项研究中，我们发现鲍曼不动杆菌和铜绿假单胞菌的中性粒细胞的反应性及其生物防御机制是不同的，这对于了解这两种细菌的致病性是重要的。(C)2014年，日本化疗学会和日本传染病协会。爱思唯尔有限公司出版。保留所有权利。
Acinetobacter baumannii and Pseudomonas aeruginosa are the same aerobic gram-negative bacillus and are usually harmless but cause infectious diseases in compromised hosts. Neutrophils play a critical role in infective protection against the extracellular growth of bacteria. Recently, a new biological defense mechanism called neutrophil extracellular traps (NETs) has been attracting attention. In present study, we investigated the responsiveness of neutrophils to A. baumannii and P. aeruginosa, focusing on NET formation. Neutrophils were co-cultured with A. baumannii or P. aeruginosa, and then DNA, histone and neutrophil elastase were stained, and the formation of NETs was evaluated. Neutrophils stimulated with A. baumannii had spread, but their shapes was maintained, and the nucleus was observed as clearly as that in non-stimulated neutrophils. However, neutrophils stimulated with P. aeruginosa did not maintain their cellular morphology, and the nucleus was disrupted with DNA, histones, and neutrophil elastase released into the extracellular space. These results suggest that A. baumannii does not induce NET formation, in contrast to P. aeruginosa. In addition, we measured expression of myeloperoxidase (MPO), reactive oxygen species (ROS) and superoxide in neutrophils, and we found that these expression in P. aeruginosa-stimulated neutrophils was stronger than that in A. baumannii-stimulated neutrophils. Furthermore, A. baumannii was not killed by neutrophils, in contrast to P. aeruginosa. In this study, we show that the reactivity of neutrophils and their biological defense mechanism are different between A. baumannii and P. aeruginosa, which is important for understanding the pathogenicity of these bacteria. (C) 2014, Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.