The effect of tumor necrosis factor α and γ-interferon on the resorption of human articular cartilage and on the production of prostaglandin e and of caseinase activity by human articular chondrocytes
The effect of tumor necrosis factor α and γ-interferon on the resorption of human articular cartilage and on the production of prostaglandin e and of caseinase activity by human articular chondrocytes
复制标题
肿瘤坏死因子α和γ-干扰素对人关节软骨吸收以及人关节软骨细胞产生前列腺素e和酪蛋白酶活性的影响
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
R. Russell
中科院分区：
文献类型：
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作者：
R. Bunning;R. Russell
在培养的人关节软骨细胞中，添加肿瘤坏死因子 α (TNF α) 可在 10(-11) M 至 10(-7) M 范围内刺激酪蛋白酶活性，并在 10(-10) M 至 10(-7) M 范围内刺激前列腺素 E (PGE) 产生。在 10(-8)M TNF α 下观察到两种活性的最大刺激。 γ-干扰素 (γ-IFN) 对 PGE 产生具有不同的影响，但在 0.1-1,000 单位/ml 的浓度范围内，对关节软骨细胞培养物中的酪蛋白酶活性没有显着影响。 TNF α 与 γ-IFN 共孵育可增强 PGE 的产生并降低酪蛋白酶活性。浓度低至 1 单位/ml 的 γ-IFN 对 TNF 刺激的 PGE 产生和酪蛋白酶活性具有显着影响。人关节软骨的吸收受TNFα(10(-7)M)刺激，并被γ-IFN(1,000单位/ml)抑制。体内软骨破坏可能是通过细胞因子之间的相互作用来调节的。
In cultured human articular chondrocytes, addition of tumor necrosis factor alpha (TNF alpha) stimulated caseinase activity over the range of 10(-11) M to 10(-7) M and stimulated prostaglandin E (PGE) production over the range of 10(-10) M to 10(-7) M. Maximal stimulation was observed at 10(-8)M TNF alpha for both activities. Gamma-interferon (gamma-IFN) had a variable effect on PGE production and no significant effect on caseinase activity in articular chondrocyte cultures over a concentration range of 0.1-1,000 units/ml. Co-incubation of TNF alpha with gamma-IFN enhanced PGE production and decreased caseinase activity. Concentrations as low as 1 unit/ml of gamma-IFN had significant effects on TNF-stimulated production of PGE and on caseinase activity. Resorption of human articular cartilage was stimulated by TNF alpha (10(-7) M) and was inhibited by gamma-IFN (1,000 units/ml). It is possible that cartilage breakdown in vivo may be modulated by such interactions between cytokines.
用重组免疫干扰素处理的人滑膜成纤维细胞增殖增加。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Brinckerhoff,CE;Guyre,PM
通讯作者：
Guyre,PM
干扰素-γ增强肿瘤坏死因子细胞受体的表达。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Tsujimoto,M;Yip,YK;Vilcek,J
通讯作者：
Vilcek,J
影响因子：
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作者：
FIRESTEIN, GS;ZVAIFLER, NJ
通讯作者：
ZVAIFLER, NJ