Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution
Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp by adaptive laboratory evolution
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DOI：
10.1016/j.biortech.2015.03.011
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发表时间：
2015-06-01
影响因子：
11.4
通讯作者：
Sun, Yuhan
中科院分区：
文献类型：
--
作者：
Li, Dengjin;Wang, Liang;Sun, Yuhan
微藻捕集CO2是一种很有前途的温室气体减排方法。因此，构建一种高效的方法来获得具有高CO2固定能力的耐高CO2微藻菌株至关重要。本研究分别在10%CO2和20%CO2条件下进行了31轮的适应性实验室进化（ALE），获得了两株进化的小球藻AE 10和AE 20。两株菌在30%CO2中生长迅速，最大生物量为3.68 ± 0.08g/L，分别是出发菌株和AE 20的1.22倍和2.94倍。在1- 30%CO2浓度下，AE 10和AE 20的叶绿素含量显著高于对照。研究了ALE工艺对小球藻细胞生化组成的影响。本研究证明ALE是提高小球藻高CO2耐受性的有效方法。（C）2015 Elsevier Ltd.版权所有。
CO2 capture by microalgae is a promising method to reduce greenhouse gas emissions. It is critical to construct a highly efficient way to obtain a microalgal strain tolerant to high CO2 concentrations with high CO2 fixation capability. In this study, two evolved Chlorella sp. strains, AE10 and AE20 were obtained after 31 cycles of adaptive laboratory evolution (ALE) under 10% and 20% CO2, respectively. Both of them grew rapidly in 30% CO2 and the maximal biomass concentration of AE10 was 3.68 +/- 0.08 g/L, which was 1.22 and 2.94 times to those of AE20 and original strain, respectively. The chlorophyll contents of AE10 and AE20 were significantly higher than those of the original one under 1-30% CO2. The influences of ALE process on biochemical compositions of Chlorella cells were also investigated. This study proved that ALE was an effective approach to improve high CO2 tolerance of Chlorella sp. (C) 2015 Elsevier Ltd. All rights reserved.