Cytokeratin subtypes in thyroid tumours: Immunohistochemical study with emphasis on the follicular variant of papillary carcinoma
Cytokeratin subtypes in thyroid tumours: Immunohistochemical study with emphasis on the follicular variant of papillary carcinoma
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DOI：
10.2310/7070.2003.11429
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发表时间：
2003-10-01
期刊：
影响因子：
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通讯作者：
Berean, KW
中科院分区：
文献类型：
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作者：
Cameron, BR;Berean, KW
目的：滤泡型甲状腺乳头状癌（FV-PTC）可能难以与其他具有滤泡结构的甲状腺肿瘤（如滤泡性腺瘤、滤泡性癌和结节性甲状腺肿中的优势结节）鉴别。这些病变在治疗和预期结果方面的明显差异要求准确区分它们。为了减少这种鉴别诊断的主观性，我们进行了这项研究，以确定是否有任何差异，在细胞角蛋白的轮廓之间存在FV-PTC和其他滤泡病变的甲状腺。设计：免疫组织化学分析的基础上回顾性病理审查。设置：温哥华总医院，温哥华，BC。方法：检索了温哥华总医院解剖病理实验室的文件，以查找典型甲状腺乳头状癌（PTC）、FV-PTC、滤泡性腺瘤、滤泡性癌、和结节性甲状腺肿对切片和报告进行了审查，并选择了确诊和足够组织的病例纳入研究。细胞角蛋白19（CK 19），20（CK 20），和7（CK 7）的单克隆抗体被施加到福尔马林固定，石蜡包埋的组织section.Results：所有PTC的情况下，包括FV-PTC，以及所有滤泡性腺瘤，滤泡性癌，结节性甲状腺肿，染色阳性CK 7，除了一个单一的滤泡性癌，CK 20阴性。CK 19修饰几乎所有PTC，包括FV-PTC，尽管染色有时是局灶性的。滤泡性腺瘤，滤泡性癌，结节性甲状腺肿的情况下，大多数是阴性或显示局灶性染色与CK 19，虽然偶尔的情况下表现出弥漫性positivity.Conclusions：CK 19强烈染色的PTC，包括FV-PTC，在弥漫性的方式。但是，与。在其他滤泡病变中看到的染色限制了其在常规诊断环境中的应用。
Objective: The follicular variant of papillary thyroid carcinoma (FV-PTC) may be difficult to differentiate from other thyroid neoplasms with follicular architecture such as follicular adenoma, follicular carcinoma, and dominant nodules in nodular goitre. Obvious differences in treatment and expected outcome between these lesions mandate that the distinction between them be made accurately. To decrease the subjectivity of this differential diagnosis, we undertook this study to determine if any difference in cytokeratin profile exists between FV-PTC and other follicular lesions of the thyroid gland.Design: Immunohistochemical analysis based on a retrospective pathology review.Setting: Vancouver General Hospital, Vancouver, BC.Methods: The files of the Vancouver General Hospital anatomic pathology laboratory were searched for cases of typical papillary thyroid carcinoma (PTC), FV-PTC, follicular adenoma, follicular carcinoma, and nodular goitre. The slides and reports were reviewed, and those cases with confirmed diagnosis and adequate tissue were selected for inclusion in the study. Monoclonal antibodies to cytokeratin 19 (CK19), 20 (CK20), and 7 (CK7) were applied to formalin-fixed, paraffin-embedded tissue sections.Results: All cases of PTC, including FV-PTC, as well as all follicular adenomas, follicular carcinomas, and nodular goitres, stained positively for CK7 and, except for a single follicular carcinoma, were negative for CK20. CK19 decorated almost all PTCs, including FV-PTC, although the staining was sometimes focal. The majority of the cases of follicular adenoma, follicular carcinoma, and nodular goitre were negative or showed focal staining with CK19, although occasional cases showed diffuse positivity.Conclusions: CK19 strongly stains the majority of PTC, including FV-PTC, in a diffuse manner. However, overlap with the. staining seen in other follicular lesions limits its utility in a routine diagnostic setting.