GENETIC STUDIES OF LAC REPRESSOR .3. ADDITIONAL CORRELATION OF MUTATIONAL SITES WITH SPECIFIC AMINO-ACID RESIDUES
GENETIC STUDIES OF LAC REPRESSOR .3. ADDITIONAL CORRELATION OF MUTATIONAL SITES WITH SPECIFIC AMINO-ACID RESIDUES
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DOI：
10.1016/0022-2836(77)90056-0
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发表时间：
1977-01-01
影响因子：
5.6
通讯作者：
MILLER, JH
中科院分区：
文献类型：
--
作者：
COULONDRE, C;MILLER, JH
The complete results of the analysis of over 5300 independently derived nonsense mutations in the lacI gene [of Escherichia coli] are presented. These were mapped and divided into specific sites. A total of 105 nonsense mutations derived from 90 different codons can be distinguished, of which several are the result of tandem double base changes induced by UV light. With the aid of results determined in a preceding paper, the majority of these mutations were assigned to points in the gene coding for specific residues in the lac repressor. This allows a detailed correlation of the physical and genetic map. Recombination studies were carried out using mutations at known sites. For crosses involving mutations separated by less than 30 nucleotides (the main object of this study), a significant lack of agreement between distance and recombination frequency was found.
