The Expression of B-Type Natriuretic Peptide After CaCl2-Induced Arrhythmias in Rats
The Expression of B-Type Natriuretic Peptide After CaCl2-Induced Arrhythmias in Rats
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CaCl2诱发大鼠心律失常后B型利钠肽的表达
DOI：
10.1097/paf.0000000000000193
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发表时间：
2016-09-01
影响因子：
1
通讯作者：
Zhu, Bao-Li
中科院分区：
文献类型：
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作者：
Cao, Zhi-Peng;Zhang, Yuan;Zhu, Bao-Li
为探讨心律失常后B型钠尿肽（BNP）的表达模式，采用免疫组化、Western印迹、实时定量聚合酶链反应和酶联免疫吸附试验等方法，检测了CaCl 2诱导的大鼠心律失常后不同时间点（0 min、10 min、30 min、1 h、3 h和6 h）BNP的表达。免疫组化结果显示，持续性心律失常大鼠心内膜BNP的表达高于心外膜。通过蛋白质印迹分析测定的BNP与GAPDH（甘油醛-3-磷酸脱氢酶）的比率在0分钟时没有变化，但在10分钟时增加，并在30分钟时达到第一个峰值（0.48 [0.03]）。短暂下降后，在6小时观察到第二个峰（0.54 [0.03]）。通过实时定量聚合酶链反应也观察到BNP信使RNA表达的类似模式。血浆BNP浓度在最初的心律失常发作后没有变化，但在注射CaCl 2后30分钟显著增加。结果表明，心律失常可引起心肌和血液中BNP水平升高，且在心律失常初期BNP信使RNA水平升高，而在心肌和血浆中BNP蛋白水平不升高，持续心律失常后两者均升高。BNP表达的升高与心律失常的严重程度和持续时间直接相关，提示心源性猝死中存在致死性心律失常。
AbstractTo investigate the patterns of B-type natriuretic peptide (BNP) expression after arrhythmia, BNP was assessed at different time points (0 minute, 10 minutes, 30 minutes, 1 hour, 3 hours, and 6 hours) in CaCl2-induced arrhythmia in rats through various methods such as immunohistochemistry, Western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay. Immunohistochemistry results showed that the expression of BNP in the endocardium was higher than that in the epicardium in rats undergoing sustained arrhythmias. The BNP-to-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) ratios determined by Western blotting analysis revealed no change at 0 minute but increased at 10 minutes and reached the first peak (0.48 [0.03]) at 30 minutes. After a brief decline, the second peak was observed at 6 hours (0.54 [0.03]). Similar patterns of BNP messenger RNA expression were also observed by quantitative real-time polymerase chain reaction. The plasma BNP concentrations did not change after initial bouts of cardiac arrhythmias but significantly increased 30 minutes after CaCl2 injections. The results demonstrate that arrhythmia causes an elevation of BNP in the myocardium and blood, and BNP messenger RNA increases in initial arrhythmia while its protein in myocardium and plasma does not; however, both of them were elevated after sustained arrhythmia. Such an elevated BNP expression, which is directly related to the severity and duration of the arrhythmias, may suggest the existence of fatal arrhythmia in sudden cardiac death.