Monoclonal antibodies against human acid alpha-glucosidase.
Monoclonal antibodies against human acid alpha-glucosidase.
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抗人酸性α-葡萄糖苷酶的单克隆抗体。
DOI：
10.1016/0304-4165(81)90041-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Hilgers
中科院分区：
文献类型：
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作者：
J. Hilkens;J. Tager;F. Buijs;B. Brouwer;G. van Thienen;F. P. Tegelaers;J. Hilgers
使用从人胎盘纯化的酸性α-葡糖苷酶根据先前描述的程序免疫小鼠（Balb/cHeA株）（Stähli，C.，Staehlin，T.，Miggiano，V.，施密特Häring，P.（1980）J. Immunol. Methods 32，297-304）。脾细胞与骨髓细胞融合后，约有10%的杂交克隆产生抗酸性α-葡萄糖苷酶的抗体。最后，获得了8个产生抗酶抗体的稳定克隆。当在十二烷基硫酸钠存在下通过聚丙烯酰胺凝胶电泳分析纯化的酸性α-葡萄糖苷酶时，两个主要的主要蛋白条带（mol.重量76 000和70 000），一个次要带的摩尔。重量96000和几个次要条带，分子量为10000。重量67000或更低。由于所有这些组分都与单克隆抗体反应，它们必须至少有一个共同的抗原决定簇。
Acid α-glucosidase purified from human placenta was used to immunize a mouse (strain Balb/cHeA) according to a procedure described earlier (Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J. and Häring, P. (1980) J. Immunol. Methods 32, 297–304). After fusion of spleen cells with myelona cells, about 10% of the hybrid clones obtained produced antibodies against acid α-glucosidase. Finally, eight stable clones producing antibodies against the enzyme were obtained. When purified acid α-glucosidase is analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, two major major protein bands (mol. wt. 76 000 and 70 000), a minor band of mol. wt. 96 000 and several minor bands with a mol. wt. of 67 000 or lower are seen. Since all these component react with the monoclonal antibodies, they must have at least one antigenic determinant in common.