GROWTH OF 2 PHLEBOVIRUSES AFTER EXPERIMENTAL-INFECTION OF THEIR SUSPECTED SAND FLY VECTOR, PHLEBOTOMUS-PERNICIOSUS (DIPTERA, PSYCHODIDAE)
GROWTH OF 2 PHLEBOVIRUSES AFTER EXPERIMENTAL-INFECTION OF THEIR SUSPECTED SAND FLY VECTOR, PHLEBOTOMUS-PERNICIOSUS (DIPTERA, PSYCHODIDAE)
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DOI：
10.4269/ajtmh.1985.34.174
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发表时间：
1985-01-01
影响因子：
3.3
通讯作者：
VERANI, P
中科院分区：
文献类型：
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作者：
CIUFOLINI, MG;MAROLI, M;VERANI, P
用从意大利分离的2种白蛉病毒（托斯卡纳和Arbia），通过胸内接种和膜饲养技术感染恶性白蛉。两种病毒的低水平的增殖检测后，胸腔内接种的白蛉。只有部分昆虫经口感染了这两种病毒。经口感染的苍蝇百分比与摄入的病毒量有关。托斯卡纳病毒经卵巢传播给经口感染白蛉的F1代的2只幼虫。当测试非天然病毒-载体组合时，在口服感染后未观察到感染迹象，托斯卡纳病毒-帕帕塔斯白蛉或那不勒斯沙蝇热病毒-恶性白蛉。实验感染托斯卡纳和Arbia病毒的P. perniciosus中记录的病毒浓度与自然感染的白蛉中发现的相似。
P. perniciosus were infected by intrathoracic inoculation and membrane feeding techniques with 2 phleboviruses (Toscana and Arbia) isolated in Italy from this sand fly species. Low levels of multiplication of both viruses were detected after intrathoracic inoculation of the sand flies. Only some insects were found infected after oral ingestion of the 2 viruses. The percentage of flies infected orally was related to the amount of virus ingested. Toscana virus was transovarially transmitted to 2 larvae of the F1 progeny of orally infected sand flies. No signs of infection were observed after oral infection when unnatural virus-vector combinations were tested, e.g., Toscana virus-P. papatasi or Naples sand fly fever virus-P. perniciosus. The virus concentrations recorded in P. perniciosus experimentally infected with both Toscana and Arbia viruses were similar to those found in naturally-infected sand flies.