EXPRESSION ON HUMAN THYMOCYTES OF THE IDIOTYPIC STRUCTURES (TI) FROM 2 LEUKEMIA T-CELL LINES JURKAT AND HPB-ALL
EXPRESSION ON HUMAN THYMOCYTES OF THE IDIOTYPIC STRUCTURES (TI) FROM 2 LEUKEMIA T-CELL LINES JURKAT AND HPB-ALL
复制标题
DOI：
10.1002/eji.1830160610
复制
发表时间：
1986-06-01
影响因子：
5.4
通讯作者：
MACH, JP
中科院分区：
文献类型：
--
作者：
CARREL, S;ISLER, P;MACH, JP
特异型结构（Ti）在大量胸腺细胞上的表达仅限于HPB-ALL或Jurkat细胞。流式微荧光法分析时，多达2-4%的胸腺细胞被抗ti HPB-ALL或抗ti Jurkat单克隆抗体染色。对冷冻的胎儿或儿童胸腺标本进行的免疫组织化学定位研究表明，在皮质和髓质中都有分散分布的ti阳性细胞。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析显示，从125i标记的儿童胸腺细胞裂解物中，抗ti HPB-ALL和抗ti Jurkat单克隆抗体沉淀的二硫连接异二聚体与125i标记的HPB-ALL或Jurkat细胞沉淀的异二聚体相当。
The expression on a significant number of thymocytes of idiotypic structures (Ti) restricted to HPB-ALL or Jurkat cells is demonstrated. As many as 2-4% of thymocytes were stained with anti-Ti HPB-ALL or anti-Ti Jurkat monoclonal antibodies, when analyzed by flow microfluorometry. Immunohistochemical localization studies performed on frozen thymus specimens of either fetal or pediatric origin indicated a scattered distribution of Ti-positive cells in both the cortex and the medulla. From lysates of 125I-labeled pediatric thymocytes, anti-Ti HPB-ALL and anti-Ti Jurkat monoclonal antibodies precipitated disulfide-linked heterodimers comparable to those precipitated from 125I-labeled HPB-ALL or Jurkat cells as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.