Localization of interleukin-1 type I receptor and interleukin-1 beta in human endometrium throughout the menstrual cycle.
Localization of interleukin-1 type I receptor and interleukin-1 beta in human endometrium throughout the menstrual cycle.
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在整个月经周期中，IL-1 I 型受体和 IL-1 β 在人类子宫内膜中的定位。
DOI：
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发表时间：
1993
影响因子：
5.8
通讯作者：
M. Polan
中科院分区：
文献类型：
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作者：
Carlos Simón;G. N. Piquette;A. Francés;M. Polan
以往的人类研究表明，白细胞介素1(IL-1)系统可能是子宫内膜局部细胞间相互作用的重要旁分泌/自分泌介质。在这项研究中，我们确定了IL-1受体I型(IL-1Rti)在腺细胞及其配体的信使RNA(MRNA)和蛋白水平上的表达，IL-1β通过免疫组织化学方法定位于整个月经周期的子宫内膜内皮细胞和分离的间质细胞。用32P标记的互补DNA和互补RNA32P标记的探针在腺上皮细胞中检测到IL-1R ti mRNA。人腺上皮在整个月经周期中含有一个5.1kb的mRNA转录本。狭缝杂交信号的定量密度分析显示，IL-1Rti在分泌早期和中晚期均高于增殖期(P<；0.05)。用间接免疫荧光法和亲和素-生物素-过氧化物酶标记法检测子宫内膜腺上皮细胞中IL-1Rti蛋白的表达。然而，与子宫内膜腺深层相比，IL-1Rti在子宫内膜上皮细胞中的表达更强。用同样的方法，在整个月经周期中，IL-1β在螺旋血管内皮细胞和分离的间质细胞中都有表达，并且从增殖期到分泌期的染色增强。人子宫内膜上皮中IL-1Rti及其配体IL-1β在分离的基质细胞和内皮细胞中的检测，是免疫系统和生殖系统之间可能存在的与人类着床特别相关的通讯的另一个例子。
Previous studies in the human suggest that the interleukin-1 (IL-1) system, may be an important paracrine/autocrine mediator in local intercellular interaction in endometrial tissue. In this study we have determined that IL-1 receptor type I (IL-1R tI) is expressed at the messenger RNA (mRNA) and protein levels in glandular cells and its ligand, IL-1 beta has been localized by immunohistochemical methods in endothelial cells and isolated stromal cells in the human endometrium throughout the menstrual cycle. IL-1R tI mRNA was detected in glandular epithelium using both specific complementary DNA and complementary RNA 32P-labeled probes. Human glandular epithelium contains a 5.1-kilobase mRNA transcript throughout the complete menstrual cycle. Quantitative densitometric analysis of slot blot hybridization signals shows an increase of IL-1R tI mRNA in both early and mid-late secretory phases in comparison with the proliferative phase (P < 0.05). IL-1R tI protein was localized in endometrial glandular epithelial cells using both indirect immunofluorescence and avidin-biotin-peroxidase methods. However, more intense staining for IL-1R tI was observed in lumenal epithelial cells compared with the staining present deep in the endometrial glands. Using the same methods, IL-1 beta was detected in endothelial cells of spiral vessels and isolated stromal cells throughout the menstrual cycle, and an increased staining from proliferative to secretory phase was observed. The detection of IL-1R tI in the human endometrial epithelium and its ligand, IL-1 beta, in isolated stromal cells and endothelial cells, is another example of possible communication between the immune and reproductive systems with special relevance to human implantation.