Determination of capsaicin and dihydrocapsaicin in Capsicum anuum and related products by capillary electrophoresis with a mixed surfactant system
Determination of capsaicin and dihydrocapsaicin in Capsicum anuum and related products by capillary electrophoresis with a mixed surfactant system
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混合表面活性剂体系毛细管电泳法测定辣椒及相关产品中的辣椒素和二氢辣椒素
DOI：
10.1016/j.foodchem.2009.08.045
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发表时间：
2010-04-01
期刊：
影响因子：
8.8
通讯作者：
Tan, Suiyi
中科院分区：
文献类型：
--
作者：
Liu, Lihong;Chen, Xingguo;Tan, Suiyi
报道了一种简便、快速、灵敏、廉价的毛细管电泳法，该方法以十二烷基硫酸钠(SID)和聚氧乙烯山梨醇单月桂酸酯(Tween20)为改进剂，在缓冲液中形成混合表面活性剂体系。对辣椒素、辣椒酱和辣椒膏中的辣椒素和二氢辣椒素进行了定量分析。经过一系列优化后，在最佳条件下(15 mM四硼酸钠-0.05%(v/v)吐温20-2.2 mM十二烷基硫酸钠缓冲液(pH 10.1)，20kV电压，214 nm紫外检测)，分析物在5min内达到基线分离。辣椒素和二氢辣椒素的线性关系良好，回归方程r(2)分别为0.9994和0.9996。辣椒素和二氢辣椒素的回收率分别为90-107%和92-109%。分别进行了分析。(C)2009爱思唯尔有限公司。保留所有权利。
An easy, rapid, sensitive, and cheap capillary electrophoresis (CE) method based a mixed surfactant system formed by sodium dodecyl sulphate (SIDS) and polyoxyethylene sorbitan monolaurate (Tween 20) as modifier in the buffer was reported. Quantitative analysis of capsaicin and dihydrocapsaicin in Capsicum anuum, pepper sauce and porous capsicum plaster was demonstrated. After conducting a series of optimisations, baseline separation was obtained for the analytes within 5 min under the optimum conditions (15 mM sodium tetra borate-0.05% (v/v) Tween 20-2.2 mM SDS buffer (pH 10.1), 20 kV voltage, 214 nm UV detection). The method resulted in excellent linearity, with r(2) of regression equation of 0.9994 and 0.9996 for capsaicin and dihydrocapsaicin, respectively. Recoveries were in the range 90-107% and 92-109% for capsaicin and dihydrocapsaicin. respectively. (C) 2009 Elsevier Ltd. All rights reserved.