INDUCTION OF RUNNING ACTIVITY BY INTRACEREBRAL IMPLANTS OF ESTROGEN IN OVARIECTOMIZED RATS
INDUCTION OF RUNNING ACTIVITY BY INTRACEREBRAL IMPLANTS OF ESTROGEN IN OVARIECTOMIZED RATS
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DOI：
10.1159/000121762
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发表时间：
1969-01-01
影响因子：
4.1
通讯作者：
SAWYER, CH
中科院分区：
文献类型：
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作者：
COLVIN, GB;SAWYER, CH
材料与方法80只雌性大鼠分为10组，每组10只，分别置于4”× 4”× 10”的活动笼中，活动笼的侧面连接有宽4”、周长30“的钢丝轮。这些大鼠最初在断奶时被放在笼子里，只有那些表现出高日常活动的大鼠才被用于激素实验。在TO R 0日龄时，切除实验动物的卵巢。在约90-95日龄时，大多数动物接受双侧脑内植入含有不同比例雌激素（苯甲酸雌二醇，EB）与胆固醇（Ch）的不锈钢管。植入物瞄准了下丘脑的各个部位。在整个研究期间的不同时间进行阴道涂片，以检查植入物中雌激素的全身扩散。尸检时记录子宫直径和肾上腺重量。
Materials and MethodsEighty female rats were employed in groups of ten that were placed individually into activity cages with a 4" x 4" x 10" living space and a wire wheel 4" in width and 30'in circumference attached to the side. The rats were initially placed in the cages as weanlings, and only those showing high daily activity were used in hormone experiments. At TO ROdays of age, experimental animals were ovariectomized. At approximately 90-95 days of age, most of the animals received bilateral intracerebral implants of stainless steel tubing containing different ratios of estrogen (estradiol benzoate, EB) to cholesterol (Ch). The implants were aimed at various hypothalamic loci. Vaginal smears were taken at various times throughout the study to check for systemic spread of estrogen from the implants. The diameter of the uterus and the weights of the adrenal glands were recorded at autopsy.