Porous decellularized trachea scaffold prepared by a laser micropore technique
Porous decellularized trachea scaffold prepared by a laser micropore technique
复制标题
激光微孔技术制备多孔脱细胞气管支架
DOI：
10.1016/j.jmbbm.2018.10.006
复制
发表时间：
2019-02-01
影响因子：
3.9
通讯作者：
Zhou, Guangdong
中科院分区：
文献类型：
--
作者：
Zhang, Yongjun;Xu, Yong;Zhou, Guangdong
组织工程技术的迅速发展为气管软骨的再生提供了新的途径。然而，目前缺乏理想的支架使得气管软骨组织工程化为三维（3-D）管状结构是一个巨大的挑战。尽管脱细胞气管基质（DTM）已成为公认的气管软骨再生支架，但由于其无孔结构，细胞难以从基质分离或穿透基质。为了解决这些问题，在当前的研究中应用了激光染色技术（LMT）来提高气管样品的孔隙率，并促进脱细胞处理和细胞向内生长。此外，在优化LMT和脱细胞处理参数后，LMT处理的DTM（LDTM）保留了其天然的管状结构，只有轻微的细胞外基质损伤。此外，与DTM相比，目前的研究表明LDTM显著提高了细胞的粘附率，具有完美的细胞生物相容性。此外，LDTM软骨形成的最佳植入细胞密度确定为1 × 10（8）个细胞/ml。实验结果表明，新型LDTM是一种理想的气管组织工程支架材料。
Rapid development of tissue engineering technology provides new methods for tracheal cartilage regeneration. However, the current lack of an ideal scaffold makes engineering of trachea cartilage tissue into a three-dimensional (3-D) tubular structure a great challenge. Although a decellularized trachea matrix (DTM) has become a recognized scaffold for trachea cartilage regeneration, it is difficult for cells to detach from or penetrate the matrix because of its non-porous structure. To tackle these problems, a laser micropore technique (LMT) was applied in the current study to enhance trachea sample porosity, and facilitate decellularizing treatment and cell ingrowth. Furthermore, after optimizing LMT and decellularizing treatment parameters, LMT-treated DTM (LDTM) retained its natural tubular structure with only minor extracellular matrix damage. Moreover, compared with DTM, the current study showed that LDTM significantly improved the adherence rate of cells with perfect cell biocompatibility. Moreover, the optimal implantation cell density for chondrogenesis with LDTM was determined to be 1 x 10(8) cells/ml. Collectively, the results suggest that the novel LDTM is an ideal scaffold for trachea tissue engineering.