beta2 microglobulin-deficient (B2m(null)) NOD/SCID mice are excellent recipients for studying human stem cell function.
beta2 microglobulin-deficient (B2m(null)) NOD/SCID mice are excellent recipients for studying human stem cell function.
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DOI：
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发表时间：
2000
期刊：
影响因子：
20.3
通讯作者：
O. Kollet;A. Peled;T. Byk;H. Ben‐Hur;D. Greiner;L. Shultz;T. Lapidot
中科院分区：
文献类型：
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作者：
O. Kollet;A. Peled;T. Byk;H. Ben‐Hur;D. Greiner;L. Shultz;T. Lapidot
人类SCID再生细胞（SRC）的定义是基于它们在NOD/SCID小鼠骨髓中再生髓系和淋巴系细胞群的功能能力。SRC在脐带血细胞中的频率为1 / 9.3 x 10(5)个单核细胞。我们报道，只有8 × 10(4)个人脐带血单个核细胞移植到NOD/SCID/B2m（null）小鼠中，导致小鼠骨髓中的多谱系分化，揭示了比NOD/SCID小鼠高11倍以上的SRC频率。此外，从初次移植的NOD/SCID小鼠骨髓中恢复的CD34（+）细胞仅2至5 × 10(3)个就足以移植继发性NOD/SCID/B2m（null）小鼠的SRC，这表明SRC可以自我更新。因此，通过NOD/SCID/B2m（null）小鼠作为受体，我们建立了一种能够移植原发性和继发性免疫缺陷小鼠骨髓的人类干细胞功能试验，提供了一种更类似于自体干细胞移植的模型。
Human SCID repopulating cells (SRC) are defined based on their functional ability to repopulate the bone marrow of NOD/SCID mice with both myeloid and lymphoid cell populations. The frequency of SRC in umbilical cord blood cells is 1 in 9.3 x 10(5) mononuclear cells. We report that as few as 8 x 10(4) human cord blood mononuclear cells transplanted into NOD/SCID/B2m(null )mice resulted in multilineage differentiation in the murine bone marrow, revealing a more than 11-fold higher SRC frequency than in NOD/SCID mice. Moreover, as few as 2 to 5 x 10(3) CD34(+) cells recovered from the bone marrow of primary transplanted NOD/SCID mice were sufficient for engrafting secondary NOD/SCID/B2m(null )mice with SRC, suggesting SRC self-renewal. Thus, by using NOD/SCID/B2m(null )mice as recipients, we established a functional assay for human stem cells capable of engrafting the bone marrow of primary and secondary transplanted immune-deficient mice with SRC, providing a model that better resembles autologous stem cell transplantation.