Gene targeting technologies in rats: ZFN, TALEN, and CRISPR
Gene targeting technologies in rats: ZFN, TALEN, and CRISPR
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发表时间：
2018
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通讯作者：
T. Mashimo
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作者：
T. Mashimo
实验室大鼠作为生物医学科学中的动物模型已被广泛使用超过150年。通过显微注射将锌指核酸酶或转录激活因子样效应核酸酶应用于大鼠胚胎是产生靶向敲除大鼠的有效基因组编辑工具。最近，成簇规则间隔短回文重复序列（CRISPR）/CRISPR相关核酸内切酶已被用作小鼠和大鼠中精确和多重基因组编辑的有效工具。本文综述了这些位点特异性核酸酶技术在大鼠遗传分析和操作中的优缺点。
The laboratory rat has been widely used as an animal model in biomedical science for more than 150 years. Applying zinc-finger nucleases or transcription activator-like effector nucleases to rat embryos via microinjection is an efficient genome editing tool for generating targeted knockout rats. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonucleases have been used as an effective tool for precise and multiplex genome editing in mice and rats. In this review, the advantages and disadvantages of these site-specific nuclease technologies for genetic analysis and manipulation in rats are discussed.