IDENTIFICATION, CLONING, AND EXPRESSION OF BOLA, AN FTSZ-DEPENDENT MORPHOGENE OF ESCHERICHIA-COLI
IDENTIFICATION, CLONING, AND EXPRESSION OF BOLA, AN FTSZ-DEPENDENT MORPHOGENE OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.170.11.5169-5176.1988
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发表时间：
1988-11-01
影响因子：
3.2
通讯作者：
VICENTE, M
中科院分区：
文献类型：
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作者：
ALDEA, M;HERNANDEZCHICO, C;VICENTE, M
一个新发现的大肠杆菌，bolA，在分钟10的遗传图谱映射的形态基因，克隆在7.2-内切酶BamHI片段，并确定其能力，以产生稳定的球形细胞过表达时。这个基因编码一种13千道尔顿的多肽。bolA+的过表达是在低拷贝数载体中实现的，该载体具有与泰特和lac启动子融合的操纵子，表明顺时针方向的转录。虽然没有任何修改的青霉素结合蛋白，观察到的形态学效果，由于bolA+的过表达被证明是依赖于一个活跃的ftsZ基因产物的存在。我们的研究结果表明，存在一种机制FtsZ介导的修改新生胞壁素的构象在隔膜形成的早期步骤。
A newly found morphogene of Escherichia coli, bolA, mapping at min 10 of the genetic map, was cloned in a 7.2-kilobase BamHI fragment and identified by its ability to produce osmotically stable spherical cells when overexpressed. This gene codes for a polypeptide of 13 kilodaltons. Overexpression of bolA+ was achieved in low-copy-number vectors with operon fusions to the tet and lac promoters, indicating a clockwise direction of transcription. While no modification of any of the penicillin-binding proteins was observed, morphological effects due to overexpression of bolA+ were shown to be dependent on the presence of an active ftsZ gene product. Our results suggest the existence of a mechanism mediated by FtsZ for modifying the conformation of nascent murein in the early steps of septum formation.