Electrical characterization of protein molecules by a solid-state nanopore
Electrical characterization of protein molecules by a solid-state nanopore
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DOI：
10.1063/1.2767206
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发表时间：
2007-07-30
影响因子：
4
通讯作者：
Li, Jiali
中科院分区：
文献类型：
--
作者：
Fologea, Daniel;Ledden, Bradley;Li, Jiali
作者测量了蛋白质在离子溶液中通过电压偏置的氮化硅纳米孔进行转运时所导致的离子电流阻断情况。通过计算电流阻断的平均幅度、持续时间以及积分，他们在单分子水平上估算了蛋白质分子的相对电荷和大小。作者测量了由pH值变化引起的牛血清白蛋白（BSA）蛋白质电荷的变化。他们还通过一种改进的化学发光分析证实了BSA分子确实穿过了纳米孔。他们证明了通过固态纳米孔测量可以区分较大的纤维蛋白原蛋白质和BSA。（C）2007美国物理学会
The authors measured ionic current blockages caused by protein translocation through voltage-biased silicon nitride nanopores in ionic solution. By calculating the mean amplitude, time duration, and the integral of current blockages, they estimated the relative charge and size of protein molecules at a single molecule level. The authors measured the change in protein charge of bovine serum albumin (BSA) protein induced by pH variation. They also confirmed that BSA molecules indeed traverse nanopores using an improved chemiluminescent analysis. They demonstrated that a larger protein fibrinogen could be distinguished from BSA by a solid-state nanopore measurement. (C) 2007 American Institute of Physics.