Variations in motility and biofilm formation of Salmonella enterica serovar Typhi.
Variations in motility and biofilm formation of Salmonella enterica serovar Typhi.
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DOI：
10.1186/1757-4749-6-2
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发表时间：
2014-02-05
期刊：
影响因子：
4.2
通讯作者：
Thong KL
中科院分区：
文献类型：
--
作者：
Kalai Chelvam K;Chai LC;Thong KL
伤寒沙门氏菌（S. Typhi）作为一种细胞内的人类病原体具有独特的特征。它仅在人类宿主中引起急性和慢性感染，并伴有多种疾病表现。伤寒沙门氏菌独特的运动性和生物膜形成能力背后的主要因素在很大程度上仍然未知。本研究的主要目的是探索和研究不同背景的伤寒沙门氏菌菌株之间的运动性和生物膜形成行为。
分别使用0.25%和0.5%的琼脂浓度进行泳动和群集运动测试；同时通过在96孔微量滴定板中培养细菌48小时来测定生物膜的形成。虽然所有伤寒沙门氏菌菌株都表现出具有平滑无特征形态的群集运动，但60株中有58株表现出具有无特征或牛眼形态的泳动。有趣的是，血源性伤寒沙门氏菌菌株比粪便源性菌株表现出显著更高的泳动性（P < 0.05），这表明泳动性可能在伤寒沙门氏菌在人类宿主中的全身性侵袭中起作用。此外，粪便源性伤寒沙门氏菌在运动性和生物膜形成行为之间呈现负相关，而在血源性菌株中未观察到这种情况。
总之，泳动和群集运动在伤寒沙门氏菌菌株中是保守的，但生物膜形成能力存在差异。不同背景的伤寒沙门氏菌菌株在这种表型上没有观察到差异。这些发现为未来研究理解这种病原体的生活方式和传播提供了警示。
Salmonella enterica serovar Typhi (S. Typhi) exhibits unique characteristics as an intracellular human pathogen. It causes both acute and chronic infection with various disease manifestations in the human host only. The principal factors underlying the unique lifestyle of motility and biofilm forming ability of S. Typhi remain largely unknown. The main objective of this study was to explore and investigate the motility and biofilm forming behaviour among S. Typhi strains of diverse background. Swim and swarm motility tests were performed with 0.25% and 0.5% agar concentration, respectively; while biofilm formation was determined by growing the bacterial cultures for 48 hrs in 96-well microtitre plate. While all S. Typhi strains demonstrated swarming motility with smooth featureless morphology, 58 out of 60 strains demonstrated swimming motility with featureless or bull’s eye morphology. Interestingly, S. Typhi strains of blood-borne origin exhibited significantly higher swimming motility (P < 0.05) than stool-borne strains suggesting that swimming motility may play a role in the systemic invasion of S. Typhi in the human host. Also, stool-borne S. Typhi displayed a negative relationship between motility and biofilm forming behaviour, which was not observed in the blood-borne strains. In summary, both swimming and swarming motility are conserved among S. Typhi strains but there was variation for biofilm forming ability. There was no difference observed in this phenotype for S. Typhi strains from diverse background. These findings serve as caveats for future studies to understand the lifestyle and transmission of this pathogen.