Carvacrol inhibits proliferation and induces apoptosis in human colon cancer cells
Carvacrol inhibits proliferation and induces apoptosis in human colon cancer cells
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DOI：
10.1097/cad.0000000000000263
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发表时间：
2015-09-01
影响因子：
2.3
通讯作者：
Sun, Hongli
中科院分区：
文献类型：
--
作者：
Fan, Kai;Li, Xiaolei;Sun, Hongli
结肠癌是世界上最常见的恶性肿瘤之一，死亡率很高。香芹酚是牛至和百里香精油的主要成分，具有抗肿瘤的特性。本文研究了香芹酚对人结肠癌细胞株HCT116和LoVo增殖和凋亡的影响，并探讨了其抗肿瘤作用的分子机制。我们发现carvacrol以浓度依赖性的方式抑制两种结肠癌细胞系的增殖和迁移。carvacrol通过降低基质金属蛋白酶-2 （MMP-2）和MMP-9的表达抑制细胞侵袭。Carvacrol处理也导致细胞周期阻滞在G2/M期，并降低cyclin B1的表达。最后，香芹酚以剂量依赖的方式诱导细胞凋亡。在分子水平上，香芹酚下调Bcl-2的表达，诱导细胞外调节蛋白激酶和蛋白激酶B （p-Akt）的磷酸化。同时，carvacrol上调Bax和c-Jun n -末端激酶的表达。这些结果表明，香芹酚可能通过线粒体凋亡途径以及MAPK和PI3K/Akt信号通路诱导结肠癌细胞凋亡。总之，我们的研究结果表明，香芹酚可能具有预防和治疗结肠癌的治疗潜力。版权所有2015威科集团有限公司版权所有。
Colon cancer is one of the most common malignancies worldwide and has a high mortality rate. Carvacrol is a major component of oregano and thyme essential oils and shows antitumor properties. Here, we investigated the effects of carvacrol on the proliferation and apoptosis of two human colon cancer cell lines, HCT116 and LoVo, and studied the molecular mechanisms of its antitumor properties. We found that carvacrol inhibited the proliferation and migration of the two colon cancer cell lines in a concentration-dependent manner. Cell invasion was suppressed after carvacrol treatment by decreasing the expression of matrix metalloprotease-2 (MMP-2) and MMP-9. Carvacrol treatment also caused cell cycle arrest in the G2/M phase and decreased cyclin B1 expression. Finally, carvacrol induced cell apoptosis in a dose-dependent manner. At the molecular level, carvacrol downregulated the expression of Bcl-2 and induced the phosphorylation of the extracellular-regulated protein kinase and protein kinase B (p-Akt). In parallel, carvacrol upregulated the expression of Bax and c-Jun N-terminal kinase. These results indicate that carvacrol might induce apoptosis in colon cancer cells through the mitochondrial apoptotic pathway and the MAPK and PI3K/Akt signaling pathways. Together, our results suggest that carvacrol may have therapeutic potential for the prevention and treatment of colon cancer. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.