Effects of GABA(B) receptors in the insula on recognition memory observed with intellicage.
Effects of GABA(B) receptors in the insula on recognition memory observed with intellicage.
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脑岛 GABA(B) 受体对认知记忆的影响
DOI：
10.1186/s12993-017-0125-4
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发表时间：
2017-04-17
期刊：
影响因子：
--
通讯作者：
Sun T
中科院分区：
文献类型：
--
作者：
Wu N;Wang F;Jin Z;Zhang Z;Wang LK;Zhang C;Sun T
孤立的功能逐渐成为认知研究中的强烈研究。
雄性sprague – dawley大鼠被随机分为对照，naCl，baclofen和cgp35348组。 ）通过RT-PCR检测到mRNA水平。
智力测试的结果表明，使用Baclofen和CGP35348在位置学习，惩罚性学习和惩罚性逆转学习方面受到了损害，在适当的逆转学习中没有明显的差异。 RT-PCR和Western印迹分析表明，与对照组相比，Baclofen组的GB1和GB2的相对表达显着增加。
岛中的Gababr表达在大鼠识别记忆的形成中起重要作用。
Insular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. GABABR has been shown to be closely related to memory formation. In the present study, we used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABABR and recognition memory. Male Sprague–Dawley rats were randomly divided into control, Sham, Nacl, baclofen and CGP35348 groups. Different testing procedures were employed using intellicage to detect changes in rat recognition memory. The expression of GABABR (GB1, GB2) in the insula of rats was determined by immunofluorescence and western blotting at the protein level. In addition, the expression of GABABR (GB1, GB2) was detected by RT-PCR at the mRNA level. The results of the intellicage test showed that recognition memory was impaired in terms of position learning, punitive learning and punitive reversal learning by using baclofen and CGP35348. In position reversal learning, no significant differences were found in terms of cognitive memory ability between the control groups and the CGP and baclofen groups. Immunofluorescence data showed GABABR (GB1, GB2) expression in the insula, while data from RT-PCR and western blot analysis demonstrated that the relative expression of GB1 and GB2 was significantly increased in the baclofen group compared with the control groups. In the CGP35348 group, the expression of GB1 and GB2 was significantly decreased, but there was no significant difference in GB1 or GB2 expression in the control groups. GABABR expression in the insula plays an important role in the formation of recognition memory in rats.