Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene
Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene
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DOI：
10.1016/j.vetmic.2015.01.018
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发表时间：
2015-04-17
影响因子：
3.3
通讯作者：
Tong, Guang-zhi
中科院分区：
文献类型：
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作者：
Chen, Jia-zeng;Peng, Jin-mei;Tong, Guang-zhi
从流产母猪血清中分离到两株新发现的猪繁殖与呼吸综合征病毒（PRRSV）河南株A10和A11。有趣的是，这两种分离物可以在原代猪肺泡巨噬细胞（PAM）中复制，但不能在MARC-145细胞中复制。基于全基因组构建系统发育树，结果表明，河南A10和A11与其他高致病性PRRSV（HP-PRRSV）毒株的亲缘关系最近。基因组序列分析表明，豫A10和豫A11与HP-PRRSV代表株JXA 1的核苷酸同源性仅为96.8%~ 97.8%。值得注意的是，首次鉴定了GP 2胞内结构域中的10个氨基酸缺失。HuN 4-F112（来自HP-PRRSV的减毒株）的全长感染性cDNA克隆用于构建在GP 2中具有相应缺失的嵌合克隆。我们发现该缺失不影响病毒在MARC-145细胞中的生长，表明PRRSV GP 2的内域可能是可变的。(C)2015 Elsevier B. V.版权所有。
Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable. (C) 2015 Elsevier B.V. All rights reserved.