Isolation and Characterization of Antimicrobial Compounds in Plant Extracts against Multidrug-Resistant Acinetobacter baumannii
Isolation and Characterization of Antimicrobial Compounds in Plant Extracts against Multidrug-Resistant Acinetobacter baumannii
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DOI：
10.1371/journal.pone.0061594
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发表时间：
2013-04-22
期刊：
影响因子：
3.7
通讯作者：
Liu, George Y.
中科院分区：
文献类型：
--
作者：
Miyasaki, Yoko;Rabenstein, John D.;Liu, George Y.
用于治疗多药耐药鲍曼不动杆菌（鲍曼不动杆菌）引起的医院感染的完全有效抗生素的选择非常有限。厚朴、马洪、冬凌草、玫瑰、中国秋楸、黄芩和chebula等植物提取物对鲍曼不动杆菌临床多重耐药菌株具有生长抑制活性。本研究采用高效液相色谱法对各植物提取物进行分离，鉴定其抑菌活性，并测定各提取物对鲍曼不动杆菌的抑菌活性。通过液相色谱/质谱分析和核磁共振谱分析，对抑制bbb40 %细菌生长的组分进行了化学结构鉴定。鉴定出6种最具活性的化合物为：玫瑰中的鞣花酸；黄芩苷；chebula中的chebulic acid、chebulic acid、corilagin和terchebulin。结果表明，该化合物对鲍曼不动杆菌的最低抑菌浓度为128 mg/mL，最低杀菌浓度为256 mg/mL。诺沃戈宁与十种抗革兰氏阴性细菌药物的联合研究表明，诺沃戈宁并没有增强本研究选择的合成抗生素的抗菌活性。综上所述，在所有已鉴定的抗菌化合物中，诺沃戈宁对耐多药鲍曼不动杆菌菌株最有效。需要进一步的研究来确定诺沃戈宁对多药耐药鲍曼不动杆菌感染的预防和治疗潜力。
The number of fully active antibiotic options that treat nosocomial infections due to multidrug-resistant Acinetobacter baumannii (A. baumannii) is extremely limited. Magnolia officinalis, Mahonia bealei, Rabdosia rubescens, Rosa rugosa, Rubus chingii, Scutellaria baicalensis, and Terminalia chebula plant extracts were previously shown to have growth inhibitory activity against a multidrug-resistant clinical strain of A. baumannii. In this study, the compounds responsible for their antimicrobial activity were identified by fractionating each plant extract using high performance liquid chromatography, and determining the antimicrobial activity of each fraction against A. baumannii. The chemical structures of the fractions inhibiting >40% of the bacterial growth were elucidated by liquid chromatography/mass spectrometry analysis and nuclear magnetic resonance spectroscopy. The six most active compounds were identified as: ellagic acid in Rosa rugosa; norwogonin in Scutellaria baicalensis; and chebulagic acid, chebulinic acid, corilagin, and terchebulin in Terminalia chebula. The most potent compound was identified as norwogonin with a minimum inhibitory concentration of 128 mg/mL, and minimum bactericidal concentration of 256 mg/mL against clinically relevant strains of A. baumannii. Combination studies of norwogonin with ten anti-Gram negative bacterial agents demonstrated that norwogonin did not enhance the antimicrobial activity of the synthetic antibiotics chosen for this study. In conclusion, of all identified antimicrobial compounds, norwogonin was the most potent against multidrug-resistant A. baumannii strains. Further studies are warranted to ascertain the prophylactic and therapeutic potential of norwogonin for infections due to multidrug-resistant A. baumannii.