Multiple forced feeding of individual sandflies.
Multiple forced feeding of individual sandflies.
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对单个白蛉进行多次强制喂食。
DOI：
10.1016/0035-9203(89)90409-4
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发表时间：
1989
影响因子：
2.2
通讯作者：
Ryan,L
中科院分区：
文献类型：
--
作者：
Ryan,L
成功强制喂养 Papatasi Phlebotomus papatasi、P. perniciosus、P. argentipes、Lutzomyia longipisand L. 雌性。 spinicrassais 报道使用 A.N. 的方法。 Alexeiev [参见医学寄生虫学，莫斯科，34：467-471（1965）]，但通过用细镊子处理活跃的苍蝇翅膀来避免麻醉剂造成的高死亡率。在初步实验中，P. papatasi最初是通过小鸡皮膜喂养去纤维的兔血-小利什曼原虫（WR309）悬浮液而感染的，随着感染的进展，由于腺胃、食道和咽部骨架区域的堵塞，它越来越无法从阿列克谢耶夫方法的微毛细血管中吸收。然后，即使保持在强制喂食位置的苍蝇用cibarium反复泵送，很少或没有血液可能移动，但利什曼原虫鞭毛虫可能仍然出现在毛细管的培养基中。这种情况第一次发生在膜饲感染后 19 天，以及果蝇感染后强制饲喂的 14 天。曾经看到鞭毛虫进入毛细管介质，表明它们自己运动而不是排出。在此之前，能够在感染后 11 至 14 天重新进食的白蛉在饱食时会在肛门飞沫中排出 1-50 条活动鞭毛。该技术已被证明可以促进一种新的方法来研究传播机制和模式、野生捕获白蛉的感染性和摄食频率以及收获排出的寄生虫以进行生化表征。
Successful forced feeding of females ofPhlebotomus papatasi, P. perniciosus, P. argentipes, Lutzomyia longipalpisandL. spinicrassais reported using the method of A.N. Alexeiev [seeMedical Parasitology, Moscow, 34: 467-471 (1965)], but obviating high mortality from anaesthetics by handling the active flies by the wings with fine forceps. In preliminary experiments,P. papatasiwhich had been initially infected by feeding via chick skin membrane on a defibrinated rabbit blood-Leishmania minor(WR309) suspension became increasingly unable to imbibe from the microcapillary of Alexeiev's method due to blockage of the proventriculus, oesophagus and pharyngeal armature regions as the infection progressed. Then, even though the flies held in the force-feeding position repeatedly pump with the cibarium, little or no blood may move butLeishmaniaflagellates may nevertheless appear in the medium in the capillary tube. This first occurred 19 days after membrane-fed infection and 14 days after infection of flies subsequently force-fed. Flagellates were once seen entering the capillary medium suggesting their own movement rather than expulsion. Prior to this, sandflies capable of re-feeding 11 to 14 days after infection expelled 1-50 motile flagellates in anal droplets while engorging. The technique has been demonstrated to facilitate a novel approach to studies on the mechanism and mode of transmission, on the infectivity and feeding frequency of wild-caught sandflies and for the harvesting of expelled parasites for biochemical characterisation.