Morphological and genetic variation in the cosmopolitan snow alga Chloromonas nivalis (Volvocales, chlorophyta) from Japanese mountainous area
Morphological and genetic variation in the cosmopolitan snow alga Chloromonas nivalis (Volvocales, chlorophyta) from Japanese mountainous area
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DOI：
10.1508/cytologia.73.91
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发表时间：
2008-03-01
期刊：
影响因子：
1
通讯作者：
Nozaki, Hisayoshi
中科院分区：
文献类型：
--
作者：
Muramoto, Kyohei;Kato, Syou;Nozaki, Hisayoshi
世界性雪腐菌Chloromonasnivalis（Chodat）Hoham & Mullet [= Scotiellarnivalis（Chodat）Fritsch]的成熟合子采自Mt.通过扫描电子显微镜（SEM）和Rubisco大亚基（rbcL）基因的单细胞测序，研究了山形县Gassan的。扫描电镜观察表明，日本样品受精卵壁至少有两种类型的凸缘（直凸缘和弯凸缘）。虽然这些类型都在雪腐镰刀菌的形态变异范围内，但通过单细胞测序，至少检测到两种类型的rbcL（large Subunit of Rubisco）基因序列。虽然rh（L）基因的信息有限（340-400 bp），但系统发育分析表明，这两个日本型和C. nivalis起源于北美，属于Chloromonassnow类群。因此，C.雪腐病可能涉及多个隐绿单胞菌。要正确地进行分类鉴定，还需要进一步观察生活史的其他阶段，并进行更详细的系统发育分析。尼瓦利斯
Mature zygotes of the cosmopolitan snow alga Chloromonas nivalis (Chodat) Hoham & Mullet [=Scotiella nivalis (Chodat) Fritsch] collected from Mt. Gassan, Yamagata Prefecture, were examined by scanning electron microscopy (SEM) and single-cell sequencing of the large subunit of Rubisco (rbcL) genes. I-lie present SEM demonstrated that Japanese samples have at least two types of flanges on the zygote wall (straight and sigmate). Although these types fell within the morphological variability of C nivalis as reported previously, at least two types of rbcL (large Subunit of Rubisco) gene sequences were detected by Using the single-cell sequencing. Although the information of the rh(L genes were limited (340-400 bp), the present phylogenetic analyses revealed that these two Japanese types and C. nivalis originating front North America belong to the lineage composed of Chloromonas snow taxa. Thus, C. nivalis might involve multiple cryptic species of Chloromonas. Further ovservations of other stages of life cycle and more detailed phylogenetic analysis are needed to reveal the correct taxonomic identifications of C. nivalis.