Chemical and histological characterization of internodes of sugarcane and energy-cane hybrids throughout plant development
Chemical and histological characterization of internodes of sugarcane and energy-cane hybrids throughout plant development
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DOI：
10.1016/j.indcrop.2023.116739
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发表时间：
2023-04-24
影响因子：
5.9
通讯作者：
Acevedo, Alberto
中科院分区：
文献类型：
--
作者：
Garcia, Jose M.;Molina, Catalina;Acevedo, Alberto
甘蔗和能源甘蔗是重要的甘蔗属植物。包含能源生产所需特征的混合动力。其茎木质纤维素可作为第二代乙醇工业的原材料，有助于加速化石燃料替代。然而，仍然需要了解这些杂交品种的节间组成和糖化作用，以优化其用途，特别是在能源甘蔗中。在这项工作中，分析了甘蔗和能源甘蔗杂交品种在四个关键发育阶段（分蘖、盛大生长、早熟和晚熟）和沿茎的几个节间位置的农艺、化学和组织学节间特征和糖化潜力。除了甘蔗杂交种中的半乳糖含量高于能源甘蔗之外，两种甘蔗在整个生长周期中的细胞壁组成相当相似。主要细胞壁成分中，结晶纤维素和基质多糖含量分别在分蘖和晚熟时达到最大。木质素集中于上部节间位置和后期发育阶段。糖化潜力向顶端节间和早期发育阶段增强，并与木质素和木糖含量呈负相关。与商业甘蔗相比，在整个生长周期中，能源-甘蔗杂交种的维管束数量和后生木质部直径较少，其木质素沉积更多地延伸至实质组织。综上所述，这些发现表明发育阶段和节间位置在很大程度上影响细胞壁的组成及其对酶促降解的抵抗力。在早期发育阶段使用甘蔗/能源甘蔗木质纤维素可能有利于第二代乙醇的生产。这种用途的新管理实践应考虑糖/乙醇生产计划下的经济、社会和环境可持续性标准。
Sugarcane and energy-cane are important Saccharum spp. hybrids that encompass desirable features for energy production. Their stem lignocellulose can be used as the raw material for the second-generation ethanol industry, contributing to accelerated fossil-fuel replacement. However, knowledge on the internode composition and saccharification of these hybrids is still needed to optimize their uses, especially in energy-cane. In this work, the agronomical, chemical, and histological internode features and saccharification potential were analyzed in sugarcane and energy-cane hybrids across four critical developmental stages (tillering, grand growth, early ripening and late ripening) and several internode positions along the stem. Except for galactose content that was higher in the sugarcane hybrid compared to the energy-cane, cell-wall composition was fairly similar in both types of cane across the growing cycle. Among main cell-wall components, maximum crystalline cellulose and matrix polysaccharides contents were observed at tillering and late ripening, respectively. Lignin concentrated towards upper internode positions and advanced developmental stages. Saccharification potential augmented towards apical internodes and early developmental stages, and negatively correlated to lignin and xylose con-tents. The energy-cane hybrid showed lower number of vascular bundles and metaxylem diameter and its lignin deposition was more extended towards the parenchymal tissue, when compared to the commercial sugarcane throughout the growth cycle. Taken together, these findings demonstrate that developmental stage and internode position largely affect cell-wall composition and its recalcitrance to enzymatic degradation. The use of sugarcane/energy-cane lignocellulose from early developmental stages could be beneficial for the second gen-eration ethanol production. Novel management practices for that usage should consider economic, social and environmental sustainability standards under a sugar/ethanol production scheme.