Femtomolar Detection of Dengue Virus DNA with Serotype Identification Ability
Femtomolar Detection of Dengue Virus DNA with Serotype Identification Ability
复制标题
DOI：
10.1021/acs.analchem.8b01802
复制
发表时间：
2018-11-06
影响因子：
7.4
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Chowdhury, Ankan Dutta;Ganganboina, Akhilesh Babu;Park, Enoch Y.
登革监测仅依赖于逆转录-聚合酶链反应（RT-PCR）类型的方法来确认登革病毒血清型;然而，由于昂贵、复杂和耗时的过程，其真实的应用受到限制。在寻找一种新的传感系统，在这里，我们已经报告了一种双向检测方法，用于登革热病毒（DENV）血清型鉴定沿着DNA定量，通过使用一类新的纳米复合物的金纳米粒子（AuNP）和氮，硫共掺杂的石墨烯量子点（N，S-GQD）。N，S-GQDs@AuNP已被用于通过使用四种染料组合探针DNA的简单荧光技术进行血清型检测，其通过共聚焦显微镜进一步验证。用亚甲蓝作氧化还原指示剂，用微分脉冲伏安法（DPV）测定了DNA的定量。本研究中获得的结果清楚地表明，N，S-GQD @AuNP可以在10（-14）至10（-6）M的浓度范围内有效地分别检测DENV的四种血清型，LOD为9.4 fM。此外，将该传感器应用于临床分离的登革病毒（DENV）DNA，并对血清型进行了定量分析，结果表明该传感器具有良好的灵敏度和灵敏度。我们希望这种简单可靠的方法可以为生物医学应用中灵敏和鲁棒的传感探针的开发铺平道路。
Dengue surveillance trusts only on reverse transcription-polymerase chain reaction (RT-PCR) type methodologies for confirmation of dengue virus serotypes; however, its real time application is restricted due to the expensive, complicated, and time-consuming process. In search of a new sensing system, here, we have reported a two-way-detection method for Dengue virus (DENV) serotype identification along with DNA quantification by using a new class of nanocomposite of gold nanoparticles (AuNP) and nitrogen, sulfur codoped graphene quantum dots (N,S-GQDs). The N,S-GQDs@AuNP has been used for serotype detection via a simple fluorescence technique using four dyecombined probe DNAs which is further validated by confocal microscopy. The quantification of the DNA has been measured by the differential pulse voltammetric (DPV) technique using methyelene blue as a redox indicator. Results obtained in this study, clearly demonstrate that the N,S-GQDs@AuNP can efficiently detect the four serotypes of DENV individually in the concentration range of 10(-14 )to 10(-6 )M with the LOD of 9.4 fM. In addition, to confirm its applicability in long chained complex DNA system, the sensor was also applied to the clinically isolated DENV DNA and showed satisfactory performances for serotype identification as well as quantification. We hope this simple and reliable method can pave an avenue for the development of sensitive and robust sensing probes in biomedical applications.