Autologous reconstitution combined with durable leukemic remission after allogeneic BMT for CML: absence of persistence of a donor-derived T cell effector population
Autologous reconstitution combined with durable leukemic remission after allogeneic BMT for CML: absence of persistence of a donor-derived T cell effector population
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DOI：
10.1038/sj.bmt.1703218
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发表时间：
2001-10-01
影响因子：
4.8
通讯作者：
Bories, D
中科院分区：
文献类型：
--
作者：
Maury, S;Belhadj, K;Bories, D
我们报告一例30岁男性慢性粒细胞白血病（CML）患者，在接受HLA表型完全相同的供者骨髓移植后，自体造血功能恢复，白血病持续缓解。从BMT后第60天起，通过VNTR PCR诊断移植物丢失。为了评估可能发挥抗白血病作用的少量供体来源的T细胞群体的存在，我们对分选的T细胞连续应用了通过荧光PCR进行嵌合体分析的灵敏过程。未检测到残留的供体T细胞。我们还回顾性地表明，一种非常敏感的实时定量PCR MRD分析方法可以预测这种情况下的复发。
We report a case of autologous hematopoietic recovery with durable leukemic remission after BMT from a phenotypically HLA-identical donor in a 30-year-old male with CML. Graft loss was diagnosed from day 60 post-BMT by VNTR PCR. To assess for the presence of a minor donor-derived T cell population that could exert an anti-leukemic effect, we serially applied a sensitive process of chimerism analysis by fluorescent PCR on sorted T cells. No residual donor T cells could be detected. We also showed retrospectively that a very sensitive method of MRD analysis by real-time quantitative PCR could have permitted prediction of relapse in this case.