THE UBIQUITIN-LIGASE SYSTEM IN TRYPANOSOMA-BRUCEI-BRUCEI
THE UBIQUITIN-LIGASE SYSTEM IN TRYPANOSOMA-BRUCEI-BRUCEI
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DOI：
10.4269/ajtmh.1993.49.545
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发表时间：
1993-11-01
影响因子：
3.3
通讯作者：
VENTULLO, RM
中科院分区：
文献类型：
--
作者：
LOWRIE, DJ;GIFFIN, BF;VENTULLO, RM
非洲锥虫布氏锥虫布氏锥虫是否利用泛素连接酶系统将8，500-道尔顿蛋白泛素与其他蛋白缀合的问题尚未研究。使用I-125标记的泛素和凝胶电泳（十二烷基硫酸钠和乙酸，尿素，Triton X-100），我们寻找的标签纳入蛋白质大于泛素，以确定泛素连接酶系统的活性在细胞质和核裂解物的细长，中间，短的血流形式的锥虫。我们目前的数据表明，有一个泛素连接酶系统的细胞溶质活动在所有三种血流形式的T。布鲁氏杆菌有迹象表明，T.布鲁氏菌在它们的胞质泛素连接酶系统活性方面不同。我们的实验表明T.布鲁氏杆菌对T.布氏杆菌可以定义三种血流形式、寄生虫和其宿主之间的差异，从而导致新的化疗策略的发展。
The question of whether the African trypanosome Trypanosoma brucei brucei utilizes a ubiquitin-ligase system to conjugate the 8,500-dalton protein ubiquitin to other proteins has not been investigated. Using I-125-labeled ubiquitin and gel electrophoresis (sodium dodecyl sulfate and acetic acid, urea, Triton X-100), we looked for the incorporation of label into proteins larger than ubiquitin to determine ubiquitin-ligase system activity in cytosolic and nuclear lysates of long slender, intermediate, and short stumpy bloodstream-form trypanosomes. We present data suggesting that there is cytosolic activity of a ubiquitin-ligase system in all three bloodstream forms of T. brucei brucei. There are indications that the three bloodstream forms of T. brucei brucei differ in their cytosolic ubiquitin-ligase system activity. Our assay showed no activity of this system in the nucleus of T. brucei brucei. Further studies on the ubiquitin-ligase system in T. brucei brucei may define differences between the three bloodstream forms, the parasite, and its host, leading to development of novel chemotherapeutic strategies.