Gene transfection by quantitatively reconstituted Sendai envelope proteins into liposomes
Gene transfection by quantitatively reconstituted Sendai envelope proteins into liposomes
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DOI：
10.1038/sj.cgt.7700421
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发表时间：
2002-02-01
影响因子：
6.4
通讯作者：
Park, YS
中科院分区：
文献类型：
--
作者：
Kim, HS;Park, YS
由仙台病毒包膜蛋白组成的融合脂质体（病毒体）已被用于动物细胞的体外和体内遗传修饰。在这项研究中，含有DNA的病毒体的仙台包膜蛋白，融合蛋白和血凝素-神经氨酸酶在脂质体囊泡中的定量重建。仙台病毒体比基于1，2-二油酰基-3-（三甲基铵）丙烷的阳离子脂质体更有效地将基因转移到培养的293转化肾细胞中。在包膜蛋白和脂质的重量比为200：1时，病毒体表现出最佳的基因转染效率。与阳离子脂质体/DNA复合物相比，仙台病毒体需要相对较短的孵育时间和更少的细胞毒性。一个月后，含有DNA的仙台病毒体的转染效率保持在70%。与通过脂质体-病毒融合制备的融合脂质体相比，这种类型的仙台病毒体相对便于制备和储存。首先，由于组分是定量配制的，因此这种类型的病毒体制剂可以为基因治疗提供进一步一致的转染。
Fusogenic liposomes (virosomes) consisting of Sendai virus envelope proteins have been utilized for in vitro and in vivo genetic modification of animal cells. In this study, the virosomes containing DNA were prepared by quantitative reconstitution of Sendai envelope proteins, fusion protein and hemagglutinin-neuramindase in liposomal vesicles. The Sendai virosomes more efficiently transferred genes into cultured 293 transformed kidney cells than 1,2-dioleoyl-3-(trimethylammonium) propane-based cationic liposomes. At 200:1 weight ratio of envelope protein and lipid, the virosomes exhibited the best efficiency of gene transfection into the cells. The Sendai virosomes required relatively a short period of incubation time and much less cytotoxic, compared to the cationic liposome/DNA complex. The transfection efficiency of the Sendai virosomes containing DNA was maintained 70% after a month. This type of Sendai virosomes is relatively convenient for preparation and storage, compared to fusogenic liposomes prepared by liposome-virus fusion. First of all, because the constituents are quantitatively formulated, this type of virosome formulation can provide further consistent transfection for gene therapy.