Dynamic visualization of thrombopoiesis within bone marrow
Dynamic visualization of thrombopoiesis within bone marrow
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DOI：
10.1126/science.1146304
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发表时间：
2007-09-21
期刊：
影响因子：
56.9
通讯作者：
von Andrian, Ulrich H.
中科院分区：
文献类型：
--
作者：
Junt, Tobias;Schulze, Harald;von Andrian, Ulrich H.
血小板是由哺乳动物骨髓（BM）中的巨核细胞（mk）产生的，其机制尚不清楚。在这里，我们描述了在完整的BM中使用多光子活体显微镜来观察小鼠血小板生成。观察到mk是将动态的前血小板样突起延伸到微血管的无根细胞。这些血管内延伸似乎被流动的血液从其跨内皮茎上剪切下来，导致外周血中出现原血小板。在体外，流体剪切可促进分化mk的前血小板生成。这些结果证实了血小板在体内形成的概念，并且与血流诱导的流体动力剪切应力是血小板形成的生物物理决定因素的可能性一致。
Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.