Serotonin transporter oligomerization documented in RN46A cells and neurons by sensitized acceptor emission FRET and fluorescence lifetime imaging microscopy
Serotonin transporter oligomerization documented in RN46A cells and neurons by sensitized acceptor emission FRET and fluorescence lifetime imaging microscopy
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DOI：
10.1016/j.bbrc.2009.01.128
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发表时间：
2009-03-20
影响因子：
3.1
通讯作者：
Nyengaard, Jens R.
中科院分区：
文献类型：
--
作者：
Fjorback, Anja W.;Pla, Patrick;Nyengaard, Jens R.
5-羟色胺转运蛋白是单胺转运蛋白家族的一员，该家族还包括多巴胺和去甲肾上腺素转运蛋白。我们用敏化受体发射荧光共振能量转移（FRET）和荧光寿命成像显微镜（FLIM）研究了SERT在HEK-MSR-239细胞、RN 46 A细胞和培养的海马神经元中的寡聚化。我们能够在细胞系以及原代培养的海马神经元中显示相同的FRET效率，表明寡聚化是细胞类型无关的。用这两种FRET方法得到的结果是非常相似的，而且，与以前的结果通过供体漂白FRET显微镜得到的一致。(C)2009 Elsevier Inc. All rights reserved.
The serotonin transporter is a member of the monoamine transporter family that also includes transporters of dopamine and norepinephrine. We have used sensitized acceptor emission fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) to study the oligomerization of SERT in HEK-MSR-239 cells, RN46A cells and in Cultured hippocampal neurons. We were able to show identical FRET efficiencies in cell lines as well as in primary cultured hippocampal neurons, demonstrating that the oligomerization is cell type independent. The results obtained with both FRET approaches are very similar and furthermore, in agreement with previous results obtained by donor bleaching FRET microscopy. (C) 2009 Elsevier Inc. All rights reserved.