Measurement of total, intracellular and surface bound cations in animal cells grown in culture
Measurement of total, intracellular and surface bound cations in animal cells grown in culture
复制标题
测量培养物中生长的动物细胞中的总阳离子、细胞内阳离子和表面结合阳离子
DOI：
10.1002/jcp.1041000203
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发表时间：
1979
影响因子：
5.6
通讯作者：
A. Rubin
中科院分区：
文献类型：
--
作者：
H. Sanui;A. Rubin
A procedure is described in which animal cells grown in culture on a dish are rapidly rinsed in situ with 0.25 M sucrose solutions for subsequent measurement of total, intracellular and rapidly exchangeable Na+, K+, Mg2+ and Ca2+ by atomic absorption spectrophotometry. Repeated rinses with CO2‐free (pH ∼7) 0.25 M sucrose solution produced essentially no loss of cellular protein or cations. One 10‐second rinse with CO2‐saturated (pH 4) 0.25 M sucrose solution removed a rapidly proton exchangeable cellular cation fraction which is interpreted as being externally (membrane) bound. Rinses with physiological electrolyte solutions are shown to produce loss of cellular protein as well as displacement of surface exchangeable cations. Thus, isotonic sucrose solution is more satisfactory than electrolytic media for rinsing cultured cells prior to measurement of cellular cations. The technique employing sucrose rinse media is very rapid and reproducible and permits measurement of total, intracellular or surface bound Na+, K+, Mg2+ and Ca2+ in the same sample.
