PURIFICATION OF ADENOSINE-DEAMINASE FROM HUMAN RED-BLOOD-CELLS
PURIFICATION OF ADENOSINE-DEAMINASE FROM HUMAN RED-BLOOD-CELLS
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DOI：
10.1080/00327488108065529
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MACQUARRIE, R
中科院分区：
文献类型：
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作者：
BUEL, E;MACQUARRIE, R
Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) was purified in high yield from human erythrocytes using a simple procedure based on 2 chromatographic steps. In the 1st step enzyme is bound to a diaminohexane-Sepharose column and eluted with salt. The 2nd step is column chromatography on Separose-bound purine riboside prepared by cross-linking the riboside to the matrix with 1,4-butanediol-diglycidyl ether. The overall purification factor was .apprx. 750,000 and the purity was at least 96%. The procedure is suitable for small sample sizes and for purification of the variant forms of the enzyme obtained from a single individual.
