SMALL-VOLUME EXTRUSION APPARATUS FOR PREPARATION OF LARGE, UNILAMELLAR VESICLES
SMALL-VOLUME EXTRUSION APPARATUS FOR PREPARATION OF LARGE, UNILAMELLAR VESICLES
复制标题
DOI：
10.1016/0005-2736(91)90295-j
复制
发表时间：
1991-01-30
期刊：
影响因子：
--
通讯作者：
HU, LR
中科院分区：
文献类型：
--
作者：
MACDONALD, RC;MACDONALD, RI;HU, LR
描述了过滤器保持器的设计和性能，该过滤器保持器使得能够方便地制备通过从多层囊泡（MLV）挤出（LUV）形成的体积高达1毫升的大的单层囊泡。 过滤器保持器可使样品在两个注射器之间来回通过，这种设计可最大限度地减少过滤器堵塞，消除LUVET制备过程中更换过滤器的需要，并将制备时间缩短至几分钟。 在电子显微镜下，急速冷冻的LUVtenin的复制品是单层的，并且相当均匀，平均直径接近用于挤出它们的过滤器的孔径。 挤出本身不会使囊泡不稳定，囊泡仅在它们在冻融时被破坏时和在大多数MLV明显转化为LUVb7时的第一次挤出期间捕获荧光染料。
The design and performance of a filter holder which enables convenient preparation of volumes of up to a milliliter of large, unilamellar vesicles formed by extrusion (LUVETs) from multilamellar vesicles (MLVs) are described. The filter holder provides for back-and-forth passage of the sample between two syringes, a design that minimizes filter blockage, eliminates the need to change filters during LUVET preparation and reduces preparation time to a few minutes. Replicas of slam-frozen LUVETs in the electron microscope are unilamellar and reasonably homogeneous with an average diameter close to the pore size of the filters used to extrude them. Extrusion per se does not destabilize the vesicles, which trapped a fluorescent dye only when they were disrupted on freeze-thawing and during the first extrusion when most of the MLVs were apparently converted to LUVETs.