The RNA of turnip yellow mosaic virus exhibits icosahedral order
The RNA of turnip yellow mosaic virus exhibits icosahedral order
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DOI：
10.1016/j.virol.2005.01.036
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发表时间：
2005-04-10
期刊：
影响因子：
3.7
通讯作者：
McPherson, A
中科院分区：
文献类型：
--
作者：
Larson, SB;Lucas, RW;McPherson, A
根据野生型芜菁黄花叶病毒晶体和冻融制备的空衣壳的结构因子幅度和实验相的差异电子密度图显示，包裹的RNA有很大一部分是二十面体分布的。四个独特的碱基配对的双螺旋RNA片段，长度为一到两圈，位于33埃和101埃半径之间，并围绕2倍或5倍二十面体轴线组织。此外，RNA的单链环会侵入五聚体和六角体的衣壳，在那里它们与衣壳内表面接触。剩余的RNA在电子密度图中看不到，它一定是这些二级结构元素之间的连接纽带，很可能是二十面体无序的。在结晶学上观察到的RNA的分布似乎与基于生化数据和二级结构分析的模型一致。(C)2005 Elsevier Inc.保留所有权利。
Difference electron density maps, based on structure factor amplitudes and experimental phases from crystals of wild-type turnip yellow mosaic virus and those of empty capsids prepared by freeze-thawing, show a large portion of the encapsidated RNA to have an icosahedral distribution. Four unique segments of base-paired, double-helical RNA, one to two turns in length, lie between 33-angstrom and 101-angstrom radius and are organized about either 2-fold or 5-fold icosahedral axes. In addition, single-stranded loops of RNA invade the pentameric and hexameric capsomeres where they contact the interior capsid surface. The remaining RNA, not seen in electron density maps, must serve as connecting links between these secondary structural elements and is likely icosahedrally disordered. The distribution of RNA observed crystallographically appears to be in agreement with models based on biochemical data and secondary structural analyses. (c) 2005 Elsevier Inc. All rights reserved.