Both Jun and Fos contribute to transcription activation by the heterodimer.
Both Jun and Fos contribute to transcription activation by the heterodimer.
复制标题
DOI：
--
复制
发表时间：
1990
期刊：
影响因子：
8
通讯作者：
S. Hirai;B. Bourachot;Moshe Yaniv
中科院分区：
文献类型：
--
作者：
S. Hirai;B. Bourachot;Moshe Yaniv
比较小鼠jun原癌基因家族的三个成员c-jun、jun B和jun D的氨基酸序列，揭示了几个同源片段。其中最C端包括亮氨酸拉链基序和一簇碱性氨基酸，之前被鉴定为DNA结合域。通过缺失分析，我们发现 N 末端区域的三个保守结构域对于 Jun 同源二聚体的反式激活至关重要。预计其中只有一种会形成酸性两亲性α螺旋。 Fos 的添加和 Jun-Fos 异二聚体的形成强烈提高了反式激活水平。单独失活的 Jun 突变体在 Fos 存在下获得部分或全部活性。这种增加很大程度上取决于 Fos C 端结构域的存在。这些结果表明，在Jun-Fos异二聚体中，Jun的N端部分和Fos的C端部分都有助于反式激活，其中后者的作用更显着。
Comparison of the amino acid sequence of the three members of the mouse jun proto-oncogene family, c-jun, jun B and jun D, reveals several homologous segments. The most C-terminal of them including a leucine zipper motif and a cluster of basic amino acids was previously identified as the DNA binding domain. By deletion analysis, we show that three conserved domains in the N-terminal region are crucial for transactivation by Jun homodimers. Only one of these is predicted to form an acidic amphipathic alpha-helix. The addition of Fos and the formation of Jun-Fos heterodimers strongly increases the transactivation level. Jun mutants that are inactive alone gain partial or full activity in the presence of Fos. This increase strongly depends on the presence of the C-terminal domain of Fos. These results show that in Jun-Fos heterodimers both the N-terminal part of Jun and the C-terminal part of Fos contribute to transactivation with a more pronounced role for the latter.