The effects of aging on molecular modulators of human embryo implantation.
The effects of aging on molecular modulators of human embryo implantation.
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DOI：
10.1016/j.isci.2021.102751
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发表时间：
2021-07-23
期刊：
影响因子：
5.8
通讯作者：
Miller D
中科院分区：
文献类型：
--
作者：
Ntostis P;Swanson G;Kokkali G;Iles D;Huntriss J;Pantou A;Tzetis M;Pantos K;Picton HM;Krawetz SA;Miller D
年龄增长对女性生育力有负面影响。随着着床率在正常母体生命过程中下降，与年龄相关的胚胎因素发生改变，我们无法在体内以无偏见的全基因组方式监测这些因素，这严重限制了我们对早期人类胚胎发育和着床的理解。我们的高通量方法使用滋养外胚层样品，代表了与滋养外胚层转录组动力学和生殖母体年龄相关的胚胎植入潜力的母体生殖年龄的全谱。潜在的胚胎-子宫内膜的相互作用进行了测试，从年轻女性的滋养外胚层采样，与接受子宫环境代表最“肥沃”的环境，成功的胚胎植入。揭示了细胞外外来体、胚胎代谢和细胞凋亡调节的潜在作用。这些生物标志物与胚胎-子宫内膜串扰/发育能力一致，作为成功着床的介导物。我们的数据为开发一种诊断测试打开了大门，用于预测接受生育治疗的女性的植入成功率。滋养外胚层转录组谱随母体年龄而改变滋养外胚层转录组谱可以描述母体生殖生物学年龄全基因组方法揭示了潜在的胚胎-子宫内膜通信可能介导着床成功的外泌体相关表达随年龄增长而减少胚胎学;组学
Advancing age has a negative impact on female fertility. As implantation rates decline during the normal maternal life course, age-related, embryonic factors are altered and our inability to monitor these factors in an unbiased genome-wide manner in vivo has severely limited our understanding of early human embryo development and implantation. Our high-throughput methodology uses trophectoderm samples representing the full spectrum of maternal reproductive ages with embryo implantation potential examined in relation to trophectoderm transcriptome dynamics and reproductive maternal age. Potential embryo-endometrial interactions were tested using trophectoderm sampled from young women, with the receptive uterine environment representing the most ‘fertile’ environment for successful embryo implantation. Potential roles for extracellular exosomes, embryonic metabolism and regulation of apoptosis were revealed. These biomarkers are consistent with embryo-endometrial crosstalk/developmental competency, serving as a mediator for successful implantation. Our data opens the door to developing a diagnostic test for predicting implantation success in women undergoing fertility treatment. Trophectoderm transcriptome profiles are altered with maternal age Trophectoderm transcriptome profiles can delineate maternal reproductive biological age A genome-wide methodology reveals potential embryo-endometrial communication Exosome-related expression that could mediate implantation success decreases with aging Embryology; Omics
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影响因子：
82.9
作者：
Cha J;Sun X;Dey SK
通讯作者：
Dey SK
DOI：
10.1073/pnas.0806219105
发表时间：
2008-10-21
影响因子：
11.1
作者：
Grewal, Seema;Carver, Janet G.;Mardon, Helen J.
通讯作者：
Mardon, Helen J.
影响因子：
64.8
作者：
Dickinson ME;Flenniken AM;Ji X;Teboul L;Wong MD;White JK;Meehan TF;Weninger WJ;Westerberg H;Adissu H;Baker CN;Bower L;Brown JM;Caddle LB;Chiani F;Clary D;Cleak J;Daly MJ;Denegre JM;Doe B;Dolan ME;Edie SM;Fuchs H;Gailus-Durner V;Galli A;Gambadoro A;Gallegos J;Guo S;Horner NR;Hsu CW;Johnson SJ;Kalaga S;Keith LC;Lanoue L;Lawson TN;Lek M;Mark M;Marschall S;Mason J;McElwee ML;Newbigging S;Nutter LM;Peterson KA;Ramirez-Solis R;Rowland DJ;Ryder E;Samocha KE;Seavitt JR;Selloum M;Szoke-Kovacs Z;Tamura M;Trainor AG;Tudose I;Wakana S;Warren J;Wendling O;West DB;Wong L;Yoshiki A;International Mouse Phenotyping Consortium;Jackson Laboratory;Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris (ICS);Charles River Laboratories;MRC Harwell;Toronto Centre for Phenogenomics;Wellcome Trust Sanger Institute;RIKEN BioResource Center;MacArthur DG;Tocchini-Valentini GP;Gao X;Flicek P;Bradley A;Skarnes WC;Justice MJ;Parkinson HE;Moore M;Wells S;Braun RE;Svenson KL;de Angelis MH;Herault Y;Mohun T;Mallon AM;Henkelman RM;Brown SD;Adams DJ;Lloyd KC;McKerlie C;Beaudet AL;Bućan M;Murray SA
通讯作者：
Murray SA
影响因子：
56.9
作者：
Gruhn, Jennifer R.;Zielinska, Agata P.;Hoffmann, Eva R.
通讯作者：
Hoffmann, Eva R.
影响因子：
5.8
作者：
Chiesa, Mattia;Colombo, Gualtiero I.;Piacentini, Luca
通讯作者：
Piacentini, Luca